4种中药微细粉体显微特征及细胞破壁率的测定

Study on Microscopic Feature and Wall-broken Rate of Four Kinds of Traditional Chinese Medicine Micro-fine Powders

目的:测定丹参、黄芩、苦参和秦艽4种常用中药微细粉体的细胞破壁率,比较其与常规细粉的显微特征差异。方法:采用细胞计数法,以木栓细胞、薄壁细胞作为计数指标,考察微细粉体技术对4种中药细胞破壁率的影响;同时采用显微观察法比较常规细粉与微粉体的细胞结构及后含物形态变化。结果:4种中药微细粉体的细胞破壁均在95%以上,且细胞多已破碎,偶见完整细胞和导管等碎片。结论:微细粉体技术对丹参、黄芩、苦参、秦艽4种中药具有良好的细胞破壁效果。

Objective：To determine the wall-broken rates of four kinds of traditional Chinese medicine （TCM） micro-fine powders,including Radix Salviae Miltiorrhizae,Radix Seutellariae,Radix Sophorae Flavescentis and Radix Gentianae Macrophyllae.Moreover,the microscopic feature differences between micro-fine powders and conventional powders were compared.Methods： The cell counting method was applied to evaluate the effect of micro-fine grinding technology on the wall-broken rate with the number of intact cork cell and parenchyma cell as the index.Meanwhile,the cell structure and ergastic substances were compared between micro-fine powders and conventional powders by microscopic observation method.Results： The wall-broken rates of four kinds of TCM micro-fine powders were over 95%.The cell wall was almost broken and the intact cell and vessel fragment were occasional.Conclusion： Micro-fine grinding technology has a good effect on the wall-broken rates of four kinds of TCM micro-fine powders,including Radix Salviae Miltiorrhizae,Radix Seutellariae,Radix Sophorae Flavescentis and Radix Gentianae Macrophyllae.