响应面法优化莲子蛋白提取工艺研究

Optimization of Extraction Process of Proteinfrom Lotus Seed by Response Surface Methodology

目的:研究莲子蛋白质水提取的最佳条件。方法:在单因素试验分析的基础上,采用合理的实验设计方案,通过响应面法优化莲子蛋白的提取条件。结果:提取莲子蛋白的最佳工艺参数为提取p H值8.0,料液比1∶15,提取时间7 h,蛋白提取率为14.53%。结论:响应面法优化的莲子蛋白提取条件简便易行,准确性和重现性好,具有实际应用价值。

Objective：To optimize the extraction process for protein from lotus seed.Methods：Based on the single factor analysis,the lotus seed protein extraction conditions were optimized by the response surface method.Results：The results showed that the optima extraction conditions were as follows：initial pH 8.0,solid-liquid ratio 1∶15,extraction time 7 h,which resulted in in an extraction yield of 14.53%.Conclusion：By using the response surface methodology extraction technology,the extraction process was easy to operate and accurate and good reproducible and has a practical application value.