响应面法优化干蟾皮的提取工艺

Optimization of Extraction Craft Bufo Corium by Using Box-Behnken Response Surface Method

目的:优选干蟾皮的提取工艺条件。方法:采用回流提取法提取干蟾皮,以华蟾酥毒基和酯蟾毒配基的总量为考察指标,运用响应面法的设计,考察乙醇浓度、液料比、提取时间对提取工艺的影响。结果:优化的提取工艺条件为乙醇浓度为88.64%,液料比为12∶1,提取时间为1.49 h,在该优化条件下,华蟾酥毒基和酯蟾毒配基的总量为2.24 mg。结论:该优化工艺简便、预测性良好,可以为干蟾皮的开发提供参考。

Objective：To optimize the extraction process of craft bufo.Methods：The refluxing process was applied,the amount of cinobufagin and resibufogenin was used as response value,box-Behnken response surface method was employed to investigate the influence of ethanol concentration,extraction time and the amount of alcohol added on the extraction process of craft bufo.Results：The optimized extraction technology was：extracted 2 times with 12 times the amount of 88.64% ethanol;1.49 hours per time.Under the optimized conditions,the total amount of cinobufagin and resibufogenin was 2.24 mg.Conclusion：The optimized extraction process is simple and predictable,can provide a reference basis for production.