摘要
为了采用响应面法对丁香提取工艺进行优化,试验以丁香提取物中丁香酚含量为考察指标,采用Box-Behnken中心组合设计方法进行提取工艺优化。结果表明:丁香提取物制备的最佳响应组合为乙醇浓度80%,提取温度59.8℃,料液比1∶11,提取时间1.68 h,经计算得到浸膏中丁香酚含量为84.39%,在此条件下验证性试验测得丁香提取物中丁香酚含量分别为79.23%、81.34%、79.34%。说明响应面优化得到的丁香提取物制备工艺稳定、可靠。
In order to apply response surface method to optimize the extraction technology of clove extract, with the content of eugenol in clove extract as the evaluation index, Box - Behnken central composite design method was used to optimize the extraction technology. The results showed that the optimal response combination of clove extract preparation were as follows：ethanol concentration 80%, extraction temperature 59.8 ℃, solid -liquid ratio 1：11, extraction time 1.68 h, which calculated the content of the extract eugenol 84.39%, and experimental content of the extract eugenol in clove extract were calculated as 79.23% ,81.34% ,79.34% under this condition. The results indicate that the extraction technology of clove extract by response surface method is stable and reliable.
基金
公益性行业(农业)科研专项项目(201303040)
关键词
丁香提取物
响应面法
提取工艺
丁香酚
优化
Clove extract
response surface method
extraction technology
eugenol
optimizition
