双贝特含量测定方法的改进

Improvement of Simfibrate Determination Method

目的:建立HPLC法测定双贝特的含量。方法:色谱柱为Diamonsil-C18(4.6μm×150mm,5μm),流动相为水—乙腈(50:50),检测波长283nm,流速1.0ml·min-1,柱温40Ⅵ;进样量20μl。结果:双贝特进样量在2.0~100μg·ml-1范围内线性关系良好(r=0.9998,n=6)平均回收率99.05%,RSD=0.91(n=9)。结论:本方法简便,准确,重复性好,可用于双贝特质量控制。

Objective： To establish an HPLC method for determination of tolmetin Simfibrate. Method： The column was Diamonsil-C18（4.6mm×150mm, 5μm）, the mobile phase was water-acetonitrile（50：50）, detection wavelength 283 nm, flow rate of1.0ml·min-1, the column temperature was 40℃; injection volume 20μl 。Result： Simfibrate injection volume good 2.0 ~ 100μg·ml-1linear relationship（r= 0.9998,n= 6） average recovery 99.7%, RSD=0.86（n = 9）. Conclusion： The method is simple, accurate,reproducible and can be used to determine tolmetin Simfibrate..