“平衡复位正骨推拿法”对兔颈源性突发性耳聋模型血液流变学指标及血浆ET、CGRP的影响

Effects of Bone-setting Manipulation on Hemorheology,ET and CGRP of Cervicogenic Sudden Hearing Loss in Model Rabbits

目的观察"平衡复位正骨推拿法"对兔颈源性突发性耳聋模型血液流变学指标及血浆内皮素(ET)和降钙素基因相关肽(CGRP)的影响,探讨其治疗颈源性突发性耳聋的可能作用机制。方法将40只大耳兔随机分为空白对照组、模型组、普通手法组和正骨手法组,每组10只。采用硬化剂注射法进行造模,造成颈源性突发性耳聋模型。空白对照组和模型组不予治疗,普通手法组与正骨手法组均予地塞米松0.47 mg/kg静滴,每日1次,3 d后减至0.23 mg/kg,再3 d停药,维生素B_14.67 mg/kg、维生素B_120.02 mg/kg肌注,每日1次,在此基础上普通手法组予常规手法治疗,正骨手法组予平衡复位正骨推拿手法治疗,每日1次,连续干预14 d。采用全自动血流变测试仪测定全血黏度、血浆黏度、红细胞压积及血沉,并检测血浆ET、CGRP含量。结果与空白对照组比较,模型组全血黏度、血浆黏度、血沉及血浆ET含量均升高,血浆CGRP含量下降(P<0.05);与模型组比较,普通手法组及正骨手法组的全血黏度、血浆黏度、血沉及血浆ET含量均下降,血浆CGRP含量均升高(P<0.05),正骨手法组对血浆ET、CGRP的改善优于普通手法组(P<0.05),而对血液流变学各指标的改善与普通手法组效果相当(P>0.05)。结论平衡复位正骨推拿法能改善血液黏稠状态,增加血流量,调节血管舒缩功能,促进听力恢复。

Objective： To observe the effects of bone-setting manipulation on hemorheology,ET and CGRP of cervicogenic sudden hearing loss in model rabbits and explore its possible mechanism. Methods： Forty big ear rabbits were randomly divided into the control group,the model group,common massage group and bone-setting manipulation group,ten rabbits in each. Cervicogenic sudden hearing loss model was duplicated with sclerosing agent injection. Rabbits in the control group and the model group received no treatment;rabbits in common massage group and bone-setting manipulation group were treated with dexamethasone intravenous drip of 0.47 mg/kg once a day,and 3 days later,decreased to 0.23 mg/kg once a day,and after 3 days,only with vitamin B_1 and vitamin B_12 intramuscular injection of 4.67 mg/kg and 0.02 mg/kg once a day. Based on this,the common massage group was treated with conventional massage and the bone-setting manipulation group was treated with bone-setting manipulation continuously for 14 days. Whole blood viscosity,plasma viscosity,hematocrit and ESR were detected with automatic blood rheometer,and the content of ET and CGRP in plasma was detected,too. Results： Compared with the control group,whole blood viscosity,plasma viscosity,ESR and the content of ET all increased in the model group,while the content of CGRP dropped（P〈0.05）. Compared with the model group,whole blood viscosity,plasma viscosity,ESR and the content of ET all dropped in common massage group and bone-setting manipulation group,while the content of CGRP increased（P〈0.05）. The improvement of ET and CGRP in bone-setting manipulation group was better than that of common massage group（P〈0.05）. However,there was no significant difference in the improvement of hemorheology indexes between them（P〉0.05）. Conclusion： The bone-setting manipulation can resist vascular spasm and improve microcirculation and the conduction of the brainstem cochlear path,which can promote hearing recovery.