2013-2015年湖南省7家生殖中心洁净实验室环境检测结果及其影响因素分析

Environment detection results and their influencing factors of clean operating laboratories in 7 reproductive centers in Hunan Province,2013-2015

目的了解湖南省生殖中心洁净实验室的卫生学状况及其主要影响因素,以促进生殖中心洁净实验环境的监测和管理。方法于2013-2015年对湖南省7家生殖中心35间洁净实验室参照《医院洁净手术部建筑技术规范》(GB50333-2013)中相应技术指标(洁净度、细菌平均浓度、换气次数、静压差、温度、湿度、噪声、工作照度)进行现场静态条件下的监测和评判。结果随着使用年限的延长,生殖中心洁净环境综合合格率呈下降趋势(χ~2=8.146,P=0.04)。使用1、2、≥3年的合格率分别为60%、42.68%、20%,检测指标中,洁净度的合格率最低(64.44%),其次为静压差(75.56%)、菌落数(81.11%)、换气次数(84.44%)、湿度(88.89%)、噪声(92.22%)、工作照度(92.22%)和温度(95.56%)。回归分析显示,细菌平均浓度(OR=29.814,95%CI:1.374~646.799,P=0.031)和换气次数(OR=59.271,95%CI:3.141~1 118.263,P=0.006)为洁净实验室环境合格率的主要影响因素。结论 2013-2015年,随着使用年份的增加,湖南省7家生殖中心洁净实验室环境合格率呈下降趋势;细菌平均浓度、换气次数为洁净实验室环境合格率的两个主要影响因素。

Objective To investigate the hygienic condition of clean operating laboratories of reproductive centers and the main influencing factors in Hunan Province so as to promote the monitoring and management of environment of clean operating laboratories. Methods During 2013-2015 in Hunan Province, we conducted a survey of technical indicators （including degree of cleanness, average concentration of airborne bacteria, ventilation rate, static differential pressure, temperature, humidity, noise and operating luminance） in 35 clean operating laboratories of 7 reproductive centers under the static condition according to Architectural Technical Code for Hospital Clean Operating Department （GB50333-2013）. Results The comprehensive qualified rates of environment of clean operating laboratories showed a decreasing tendency with the increasing use life of the laboratories （χ^2=8.146, P=0.04）. The environmental qualified rates of 1 year, 2 and ≥3 years of use life were 60%, 42.68% and 20% respectively. The qualified rate of degree of cleanness was the lowest （64.44%）, followed by static differential pressure （75.56%）, average concentration of airborne bacteria （81.11%）, ventilation rate （84.44%）, humidity （88.89%）, noise （92.22%）, operating luminance （92.22%） and temperature （95.56%）. Regression analysis showed that the average concentration of airborne bacteria （OR=29.814, 95.0%CI：1.374-646.799, P=0.031） and ventilation rate （OR=59.271, 95.0%CI：3.141-1,118.263, P=0.006） were the main factors influencing the environmental qualitied rate of the clean operating laboratories. Conclusions The environmental qualitied rates of clean operating laboratories of 7 reproductive centers in Hunan Province during 2013-2015 were decreased with the increasing use life of the laboratories. The average concentration of airborne bacteria and ventilation rate are the major factors impacting the environmental qualified rates of the clean operating laboratories in this investigotion.