福建省2013年食源性金黄色葡萄球菌spa基因分型特征

Characteristics of spa genotyping of foodborne Staphylococcus aureus in Fujian Province,2013

目的对福建省食源性金黄色葡萄球菌分离株进行spa基因分型,掌握该分型技术和了解型别特征,为预防食源性疾病的暴发提供溯源方法和基础数据。方法对2013年福建省食品安全风险监测中从不同种类食品所分离的30株金黄色葡萄球菌采用PCR方法扩增A蛋白基因(spa)的X区域,序列信息通过spa基因分型国际官网(http://www.ridom.de/spaserver/)数据库,在起始端和末端保守序列之间查找不同串联重复序列的排列方式确定型别。如果数据库中无可以比对的型别,则将双向测序的结果和测序峰图上传官网,通过负责官网数据的专家确认是否为新的型别,并给出新的型别号。结果 30株金黄色葡萄球菌分为19个spa基因型,分别是t091(6株)、t14534(2株)、t899(2株)、t701(2株)、t1376(2株)、t189(2株)、t306(2株)、t14752、t286、t002、t14475、t4445、t085、t377、t2883、t2310、t796、t127和t14867,标准株ATCC6538的型别为t3297。其中,t14534、t14752、t14867这3个型为新发现的spa基因型。结论福建省2013年所分离的30株食源性金黄色葡萄球菌的spa基因型主要以t091为主。

Objective To determine the spa genotyping of foodborne Staphylococcus aureus strains in Fujian Province, to master the genotyping technique and investigate the features of the genotypes so as to provide traceability method and basic data for preventing foodborne disease outbreaks. Methods spa types of 30 Staphylococcus aureus strains isolated from different kinds of food in food safety risk monitoring in Fujian Province in 2013 were identified by the established polymorphism genotyping method. The X region of spa gene was amplified by PCR and the PCR products were sequenced. The spa types were determined on the official website （http：//www.ridom.de/spaserver/）. If the type did not have a match in the database, the result of bi-directional sequencing map was uploaded to the official website, confirmed by experts whether it was novel and then given an ID for the new type. Results Among the 30 strains of Staphylococcus aureus, 19 different spa types were identified, including t091 （6 strains）, t14534（2 strains）, t899（2 strains）, t701（2 strains）, t1376（2 strains）, t189（2 strains）, t306（2 strains）, t14752, t286, t002, t14475, t4445, t085, t377, t2883, t2310, t796, t127 and t14867. The type of the standard strain （ATCC6538）was t3297. And t14534, t14752 and t14867 were the novel spa genotypes in this study. Conclusions The main spa genotype of the 30 foodborne Staphylococcus aureus strains isolated in Fujian Province in 2013 was t091.