芦笋总皂苷对人乳腺肿瘤细胞MCF-7体外增殖的影响

Effects of Total Saponins from Asparagus Officinalis L on Proliferation of Human Breast Cancer MFC-7 Cell in Vitro

为了研究芦笋总皂苷对人乳腺肿瘤细胞MCF-7体外增殖的影响,利用超声波辅助法提取芦笋总皂苷,MTT法检测芦笋总皂苷对人乳腺肿瘤细胞MCF-7的药物敏感性.结果表明,质量浓度为150,350 mg/L的芦笋总皂苷对乳腺肿瘤细胞的增殖具有促进作用;质量浓度750mg/L时,具有抑制作用,抑制率为19.46%,之后抑制率随质量浓度增加而增加;质量浓度1 550mg/L,抑制作用最大,抑制率为54.14%.说明低质量浓度的芦笋总皂苷对人乳腺肿瘤细胞MCF-7的体外增殖具有促进作用,高质量浓度具有抑制作用并呈剂量依赖性.

The effect of total saponins from Asparagus officinalis L on proliferation of human breast cancer MCF-7 cell in vitro was studied. The total saponins were extracted from Asparagus officinalis L using the method of ultrasound-assisted extraction and the drug sensitivity of human breast cancer MCF- 7 cell were determined by MTT assay. When the concentrations of total saponins from Asparagus offici- nalis L were 150 mg/L and 350 mg/L,the proli[eration of breast cancer cell was promoted. When at 750 mg/L,the proliferation of breast cancer cell was inhibited and the inhibition rate was 19.46% ,and the in- hibition rate with the increase of concentration. At 1 550 mg/L,the inhibition rate is the largest, reach- ingn 54.14%. It can be concluded that when the total saponins from Asparagus officinalis L are in low concentration,it can promote the proliferation of human breast cancer MCF-7 cell in vitro~ however,high concentration has inhibitory effect and is in a dose-dependent manner.