锌α2糖蛋白与高尿酸血症的相关性研究

Correlation between zinc-α2-glycoprotein and hyperuricemia

目的目前锌α2糖蛋白(ZAG)与高尿酸血症(HUA)的相关性研究较少。文中旨在通过检测ZAG的mRNA、蛋白表达量在桂西地区HUA人群和尿酸(SUA)正常的健康人群之间的表达差异,初步探讨ZAG与HUA的相关性。方法收集2016年3月至2016年5月右江民族医学院附属医院HUA患者74例为HUA组,选取同期74例体检中心健康体检者作为对照组。将受试者的血液标本进行血清制备及生化指标检测、总RNA提取,采用实时荧光定量PCR检测ZAG mRNA表达量,酶联免疫吸附测定检测血清ZAG蛋白表达量,进而分析2组ZAG的mRNA和蛋白表达差异。结果 HUA组血清ZAG mRNA、ZAG蛋白表达量明显高于对照组[(13.201±3.433)vs(11.731±4.033);(6.163±1.779)vs(5.540±0.573),P<0.05]。SUA与ZAG蛋白表达量(r=0.176)、BMI(r=0.161)、SBP(r=0.144)、DBP(r=0.250)、BUN(r=0.461)、CREA(r=0.649)、TG(r=0.212)呈正相关,与HDL(r=-0.333)呈负相关,均有统计学意义(P<0.05)。结论 ZAG可能成为预防及治疗HUA及其相关代谢性疾病的新靶点。

Objective Few reports are seen about the relationship of zinc-α2-glycoprotein （ZAG） with hyperuricemia （HUA）. This study aimed to explore the correlation between ZAG and HUA by detecting the mRNA and protein expressions of ZAG in patients with HUA and in healthy subjects with normal uric acid （NUA）. Methods We enrolled 74 HUA patients and another 74 NUA subjects in this study. We collected blood samples for determination of the mRNA and protein expressions of serum ZAG by realtime PCR and ELISA respectively, followed analysis of the differences in the expressions of ZAG mRNA and protein between the two groups. Results Compared with the NUA subjects, the HUA patients showed significantly increased expressions of ZAG mRNA （11.731±4.033 vs 13.201±3.433, P〈0.05） and ZAG protein （5.540±0.573 vs 6.163±1.779, P〈0.05）. The level of serum uric acid was correlated positively with the expressions of ZAG （ r=0. 176）, BMI （r= 0.161）, SBP （ r= 0.144）, DBP （r= 0.250）, BUN （ r = 0.461）, CREA （r=0.649）, and TG （r=0.212）, but negatively with HDL （r=-0.333）, all with statistically significant differences （P〈0.05）. Conclusion The expressions of ZAG mRNA and protein can be used as a new target for prevention and treatment of HUA and its related metabolic diseases.