血小板衍生生长因子C对体外培养的人卵巢透明细胞癌细胞的增殖和趋化作用

Effect of platelet-derived growth factor-C on proliferation and migration of ovarian cancer cells in vitro

目的观察血小板衍生生长因子-C(platelet-derived growth factor-C,PDGF-C)对体外培养的人卵巢透明细胞癌(ovarian clear cell carcinoma,OCCC)ES-2细胞的增殖和趋化作用。方法体外培养ES-2细胞,添加不同浓度的PDGF-C进行干预,应用CCK8试剂盒法评价细胞增殖情况,流式细胞术检测细胞周期,利用Transwell细胞迁移实验、细胞划痕实验测定细胞迁移能力。结果与未加PDGF-C的对照组相比,PDGF-C能明显刺激ES-2细胞的增殖,在第4天增殖达高峰,PDGF-C促增殖最佳作用浓度是20μg/L;经不同浓度PDGF-C的刺激,处于细胞周期G1期的ES-2细胞比例较对照组明显降低(P<0.05),处于S期的ES-2细胞比例显著升高(P<0.05),而处于G_2+M期的细胞比例则无明显变化(P>0.05)。同时,PDGF-C提高了ES-2细胞的迁移能力。结论 PDGF-C可促进体外培养的ES-2细胞的增殖、迁移能力。

Objective To examine the effect of platelet-derived growth factor-C（ PDGF-C） on ovarian clear cell carcinoma cells ES-2 in vitro. Methods ES-2 cells were cultured with the supplement of different concentrations of PDGF-C（ 0,10,20 and 40μg /L）. CCK8 assay was used to detect the effect of PDGF-C on cell proliferation. Flow cytometry was used to detect cell cycle phases.Transwell assay and cell scratch test were used to detect cell migration ability. Results PDGF-C could not only significantly induce proliferation of ES-2 compared with the blank control group without PDGF-C,but increase the percentage of the cells in S phase. Additionally,it was found the best concentration of PDGF-C to promote the proliferation of ES-2 cells was 20 μg / L. With the stimulation of different concentrations of PDGF-C,the proportion of ES-2 cells in G1 and S cell cycle was significantly decreased（ P〈0. 05） and significantly increased（ P〈0. 05） respectively,while the proportion of cells in G2＋ M phase did not change compared with the control group（ P〉0. 05）. At the same time,PDGF-C increased the migration ability of ES-2 cells. Conclusions PDGF-C can promote the proliferation and migration of ES-2 cells in vitro.