护骨胶囊对去卵巢大鼠钙相关激素和性腺激素影响的研究

The effect of Hugu capsules on calcium related hormones and sex hormones in ovariectomized rats

目的观察护骨胶囊(Hugu capsules,HG)对去卵巢大鼠血清中与钙代谢直接相关的激素和性激素及子宫的影响。方法将60只SD雌性大鼠随机分为假手术组(Sham)、去卵巢组(OVX)、仙灵骨葆胶囊组(Pos)和护骨胶囊低、中、高剂量组(HG-L、HG-M和HG-H)。大鼠卵巢切除后,隔天开始,连续灌胃3个月。处死大鼠,取大鼠子宫与血清,观察HG对子宫重量,血清1,25双羟维生素D3(1,25(OH)2D3)、甲状旁腺激素(PTH)、雌二醇(E2)、孕酮(P)、睾酮(T)变化及子宫内膜组织的影响。结果与Sham组比:OVX组的PTH(P<0.01)和T(P<0.05)升高,E2(P<0.01)和P(P<0.05)降低;子宫重量显著减少,子宫内膜萎缩。与OVX组比:PTH在HG各个剂量组都降低,在Pos组无变化;E2在HG-L和HG-M组显著增加,在Pos组无变化;P在HG-L组降低,在Pos组显著升高;T在HG-L和HG-H组无变化,HG-M组降低,而Pos组显著升高;HG各个剂量组和Pos组的子宫重量和子宫内膜较OVX组均无明显差异。结论护骨胶囊对OVX大鼠的作用是调节PTH和E2水平,此调节作用与仙灵骨葆胶囊的作用机制不一样。护骨胶囊在补充雌激素的同时对子宫的影响不大,为推广护骨胶囊临床防治绝经后骨质疏松提供了实验依据。

Objective To observe the effects of HG on hormones related to calcium metabolism,sex hormones and uterus in ovariectomized（ OVX） rats. Methods Sixty female SD rats were distributed randomly into 6 groups： Sham group,OVX group,Xianlinggubao group（ positive control） and HG low,medium and high dose groups（ Sham,OVX,Pos,HG-L,HG-M,HG-H）.After ovariectomy,from the next day the rats were ig administrated medicine continuously for three months. After euthanizing the experimental rats,uterus and serum were taken to study the effects of HG on uterus weight,serum 1,25（ OH）2D3,PTH,E2,P and T and its influence on endometrial tissue. Results Compared with the Sham group,OVX group’s PTH（ P 〈 0. 01） and T（ P 〈0. 05） increased; E2（ P 〈 0. 01） and P（ P 〈 0. 05） decreased,uterine weight decreased significantly,and endometrial atrophy occurred. Compared with the OVX group,PTH were significantly lower in all doses of HG,but not in the Pos group; E2 increased significantly in the HG-L and HG-Mgroups,but not in the Pos group. P in the HG-L group decreased（ P 〈 0. 05）,but significantly increased in the Pos group. T in HG-L and HG-H groups was unchanged,decreased in the HG-Mgroup,but increased significantly in the Pos group. There were not significant differences between each HG dose group and Pos group in uterine weight and endometrial tissue. Conclusion The effect of Hugu capsules on OVX rats was regulating PTH and E2 levels. This regulation is different from the mechanism of Xianlinggubao. Hugu capsules,as it increased estrogen levels but had little effect on the uterus.This study provided an experimental basis for promoting Hugu capsules in clinical treatment of postmenopausal osteoporosis.