分子标记辅助回交选育高油酸花生新种质

Development of high oleic acid peanut from molecular marker assisted selection

为培育适应山东省以及黄淮海区域生产的高产、高油酸花生新品种,以山东优异花生品种花育23号(HY23)和花育31号(HY31)为母本,以高油酸花生品种Sunoleic95R、DF12和开农176(KN176)为父本,配制了3个杂交组合HY23×DF12(SAAS-01)、HY31×KN176(SAAS-02)以及HY31×Sunoleic95R(SAAS-03),在3年时间内,进行了一次杂交、4次回交和1次自交,利用优化的CAPS和PCR产物测序法,以及新开发的花生FAD2基因KASP检测方法,对自交和回交后代进行检测。SAAS-01、SAAS-02和SAAS-03三个组合分别获得了FAD2A和FAD2B隐性纯合的高油酸基因型BC4F2种子1,18和26粒;另外还获得了一批基因型为Aabb、aa Bb、Aa Bb的BC_4F_2种子,这三种基因型的杂交后代可通过进一步自交获得纯合的高油酸材料。本研究获得了遗传背景为花育23号和花育31号的高油酸花生材料,为培育适合山东省种植的高产、高油酸花生新品种奠定了基础。

To breed high oleate peanut cultivars suitable for Shandong and Huang- Huai- Hai region of China,HY23 × DF12（ SAAS- 01）,HY31 × KN176（ SAAS- 02） and HY31 × Sunoleic95 R,3 cross combinations were made using two elite peanut cultivars Huayu 23 and Huayu 31 as female parents,and 3 high oleate peanuts Sunoleic95 R,DF12 and Kainong176 as male parents. One cross,4 backcrosses and 1 selfing were carried out within 3 years. The progenies of each cross were examined by CAPS（ cleaved amplified polymorphic sequence）and sequencing of PCR products. KASP（ kompetitive allele specific PCR） was performed for more efficient examining of BC_4F_2 genotypes. The numbers of BC_4F_2 seeds with aabb genotype of three combinations SAAS- 01,SAAS- 02 and SAAS- 03 were 1,18 and 26,respectively. Different numbers of BC_4F_2 seeds with Aabb,aa Bb and Aa Bb genotypes were obtained. These seeds could be grown and selfed to obtain the aabb homozygous. These materials could provide useful resources for breeding of high oleic acid peanut cultivars with high yield and suitable for Shandong and Huang- Huai- Hai region.