骨桥蛋白在子宫内膜癌组织中的表达及对预后的影响

Expression of OPN in tissue of the paitents with endometrial carcinoma and effect on the prognosis

目的检测骨桥蛋白(osteopontin,OPN)在子宫内膜癌(endometrial carcinoma,EC)组织中的表达情况,分析探讨OPN与EC临床病理因素(年龄、手术-病理分期、组织学分化、肌层浸润深度、组织学类型、淋巴结转移)的关系及其对预后的影响。方法选取171例经病理检查确诊的EC患者,采用免疫组织化学SP法检测EC蜡块中OPN的表达,并探讨其与EC预后的关系。结果 OPN在171例EC组织中的阳性表达率为63.74%(109/171),不同手术-病理分期、组织学分化、肌层浸润深度、淋巴结转移组织中阳性表达率比较,差异有统计学意义(P<0.05)。Kaplan-Meier分析显示,OPN表达、手术-病理分期、肌层浸润深度、淋巴结转移与EC生存率有关(P<0.05)。Cox分析显示,OPN和手术-病理分期、肌层浸润深度均为EC不良预后的危险因素。结论 OPN为EC预后的危险因素,对判断预后及指导治疗具有重要的临床意义。

Objective To detect the expression of osteopontin（ OPN） in tissues of endometrial carcinomas and to analyze its relationship with clinicopathologic features（ age,clinical and surgical stage,histo-differentiation,myometrial invasion, histological type, lymph node metastasis） in order to investigate the effect on the prognosis of endometrial carcinoma. Methods One hundred and seventyone patients by pathological examination to be endometrial carcinoma were selected. The expression of OPN was identified by immunohistochemically SP method in 171 paraffin block. The relationship on the OPN expression and the prognosis of endometrial carcinoma was discussed. Results The positive expression rate of OPN was 63. 74%（ 109 /171） in 171 patients with endometrial carcinoma. The differences of OPN positive status with different situation of clinical and surgical stage, histodifferentiation,myometrial invasion and lymph node metastasis were statistical significance（ P〈0. 05）.Kaplan-Meier method showed that OPN,clinical and surgical stage,myometrial invasion and lymph node metastasis were related with survival rate of endometrial carcinoma（ P〈0. 05）. Cox analysis showed that OPN,clinical and surgical stage, myometrial invasion were risk factors of endometrial carcinoma prognosis. Conclusion OPN was an important factor effect on prognostic of endometrial carcinoma,and it had a great clinical significance in judging prognostic and guiding clinical treatment of endometrial carcinoma.