ABCG2表达水平与乳腺癌术后蒽环类药物化疗疗效和耐药性的关系

Relationship between ABCG2 expression and chemotherapeutic efficacy and drug resistance of anthracyclines after breast cancer surgery

目的 探讨三磷酸腺苷结合转运蛋白G超家族成员2（adenosine triphosphate-binding cassette superfamily G member 2,ABCG2）的m RNA和蛋白表达水平与乳腺癌患者化疗疗效和耐药性的关系。方法 选取确诊为乳腺癌的患者24例作为研究对象,所有患者均经手术治疗后辅以蒽环类药物化疗,根据WHO疗效评价标准评估患者化疗后效果和耐药性,根据化疗效果将患者分为有效组、稳定组及进展组;分别于化疗前后利用q PCR和ELISA法检测患者乳腺组织中ABCG2的m RNA和蛋白表达水平。结果 化疗后总有效率为41.67%,即仍有58.33%的患者出现耐药性;化疗后患者ABCG2 m RNA和蛋白表达水平均显著高于化疗前（P〈0.05）;化疗有效组的患者ABCG2 m RNA和蛋白表达水平显著低于化疗稳定组及进展组的患者（P〈0.05）;化疗稳定组患者ABCG2 m RNA和蛋白表达水平显著低于进展组（P〈0.05）。结论ABCG2的表达水平与乳腺癌患者化疗疗效和耐药性相关,ABCG2表达水平越高,促进药物排泄的能力越强,肿瘤细胞的耐药性越显著,化疗疗效越差。

Objective To investigate the relationship between adenosine triphosphate-binding cassette superfamily G member 2 （ABCG2） mRNA and protein expressions and chemotherapeutic efficacy and drug resistance in breast cancer patients. Methods 24 patients with breast cancer were selected as study subjects, all patients were treated with anthracycline chemotherapy after chemotherapy, according to WHO criteria, the patients were divided into effective group, stable group and advanced group according to chemotherapy effect. The levels of ABCG2 mRNA and protein in the breast tissue were detected by qPCR and ELISA before and after chemotherapy. Results After chemotherapy, the total effective rate was 41.67%, 58.33% of patients still developed drug resistance. The expressions of ABCG2 mRNA and protein in patients after chemotherapy were significantly higher than those before chemotherapy （ P〈0.05）. The expressions o f ABCG2 mRNA and protein were significantly lower in the effective group than in the stable group and advanced group （ P 〈0.05）. The expressions o f ABCG2 mRNA and protein in the stable group was significantly lower than that in the advanced group （P〈0.05）. Conclusion The expression level o f ABCG2 was correlated with the chemotherapeutic efficacy and drug resistance of breast cancer patients. The higher the ABCG2 expression level, the stronger the ability of promoting the excretion of drugs, the more significant the drug resistance of tumor cells and the worsen the chemotherapy effect.