血浆血红素氧化酶水平与2型糖尿病关系的临床研究

Correlation of plasma heme oxygenase-1 level and type 2 diabetes mellitus

目的 探讨血浆血红素氧化酶（heme oxygenase-1,HO-1）水平与2型糖尿病（diabetes mellitus type 2,T2DM）关系。方法 选取口服糖耐量试验的门诊T2DM患者和体检中心健康个体,分为T2DM组和健康对照组,对比分析2组资料中HO-1与丙二醛（methane dicarboxylic aldehyde,MDA）、细胞内活性氧平均荧光强度（mean fluorescence intensity,MFI）、稳态胰岛素评价指数（homeostasis model assessment of insulin resistance,HOMA-IR）、空腹血糖（fasting blood glucose,FBG）指标的差异,并分析HO-1与活性氧（reactive oxygen species,ROS）MFI、MDA、HOMA-IR、FBG的相关性。结果 T2DM组中的MDA、MFI、HO-1表达率均明显高于健康对照组（P〈0.05）,HO-1与MFI、MDA呈正相关（r=0.489、0.763,P〈0.05）,T2DM组中的HO-1、HOMA-IR和FBG水平均明显高于健康对照组（P〈0.05）,HO-1表达与HOMA-IR和FBG水平呈正相关（r=0.271、0.426,P〈0.05）。结论 T2DM患者在高糖状态机体处于氧化应激状态,血浆HO-1表达明显增高,HO-1与糖尿病氧化应激、胰岛素抵抗、血糖升高存在关联,对T2DM的临床病情评估以及疗效判定具有一定的意义。

Objective To investigate the correlation of plasma heme oxygenase-1 （HO-1） level and type 2 diabetes mellitus （T2DM）. Methods The outpatient with type 2 diabetes mellitus （T2DM） undergoing oral glucose tolerance test and healthy individuals with physical examination were divided into T2DM group and healthy control group, the differences between the two groups in HO-1 and methane dicarboxylic aldehyde （MDA）, mean fluorescence intensity （MFI）, homeostasis model assessment of insulin resistance （HOMA-IR） and fasting blood glucose （FBG） were comparatively analysed , and analyzed the correlations between HO-1 and reactive oxygen species （ROS） MFI, MDA, HOMA-IR and FBG. Results The type 2 diabetes group in MDA and MFI, the expression rate of HO-1 were higher than those of control group （P〈0.05）, correlation analysis of expression of HO-1 was positively correlated with MFI, MDA （f=0.489, 0.763, ^O.OS） in the T2DM group, HO-1, HOMA- IR and FBG were significantly higher than the healthy control group, the difference was statistically significant（P〈0.05）. The expression of HO-1 and HOMA-IR and FPG levels were positively correlated in the T2DM group （r=0.271, 0.426, P 〈0.05）. Conclusion T2DM patients with hyperglycemia and oxidative stress, plasma HO-1 expression is significantly increased, HO-1 is related to oxidative stress, insulin resistance and hyperglycaemia, which has certain value on clinical assessment of T2DM and therapeutic efficacy.