灵芝多糖Fe(Ⅲ)配合物的合成及吸附动力学研究

Study on synthesis and adsorption kinetics of Ganoderma lucidum Polysaccharide-Fe（Ⅲ） complex

目的提取灵芝多糖,确定灵芝多糖Fe（Ⅲ）配合物的合成条件,并研究其吸附动力特性。方法采用水提、醇沉、脱色、透析等方法,提取灵芝多糖,在碱性条件下,灵芝多糖水溶液与FeCl_3反应,合成灵芝多糖铁配合物（GPC）;采用邻菲罗啉分光光度法,分析GPC中Fe（Ⅲ）在不同条件下的含量变化,确定灵芝多糖对Fe（Ⅲ）的吸附动力学特性;通过红外光谱对其结构进行分析。结果所制得的GPC为红棕色无定型粉末,易溶于水,在pH值3～12范围内不沉淀、不水解、不溶于有机溶剂;在相同温度下,灵芝多糖对Fe（Ⅲ）的吸附量随时间逐渐增大,于1.5 h后基本达到稳定;在相同时间,灵芝多糖对Fe（Ⅲ）的吸附量随温度的升高而增大。红外光谱检测分析证明灵芝多糖可与金属离子铁（Ⅲ）生成稳定的配合物,发生配合的基团主要是羟基,其中铁以聚合的β-FeOOH铁核结构存在于GPC中。结论灵芝多糖作为复合体与金属铁离子结合能形成稳定的GPC,时间和温度是影响GPC制备工艺的关键条件;GPC有望开发成为一种新型理想的口服补铁剂。

Objective To confirm the synthesis conditions of Ganoderma lucidum Polysaccharide-Fe（Ⅲ） Complex,and study on adsorption kinetics of it.Methods Adopt alcohol sedimenting technique and decoloring,dialysis,etc,extracting refined polysaccharide from Ganoderma lucidum.The solution of the Polysaccharide with FeCl_3 react in alkaline conditions.To synthesize the polysaccharide iron complexes（GPC）.The iron contents were determined by o-phenanthroline spectrophotography and and study on adsorption kinetics of Ganoderma lucidum Polysaccharide-Fe（Ⅲ）Complex.Results GPC is red brown and shapeless powder,it＇s liable to dissove in water,its solution is neutral,without precipitation,dissociation and Fe^（3＋） at the pH from 3 to 12,and insoluble in organic solvents.So GPC is very stable.At the same time under different temperatures,the Fe^（3＋） and polysaccharide on the adsorption quantity of increased gradually over time,the basic stability after 1.5 h.At the same time,with different temperatures the adsorption quantity of polysaccharide Fe3＋ increases with the rise of temperature.Conclusion GPC may become a new ideal oral iron.Time and temperature are the key fabrication processes of GPC.