深圳地区2015年婴幼儿腹泻轮状病毒分子流行病学调查

Investigation on molecular epidemiology of rotavirus in diarrhea infants in 2015,Shenzhen

目的调查深圳地区2015年1月-12月致婴幼儿腹泻A组轮状病毒的分子流行病学特征及病毒基因亚型特点。方法收集深圳市2015年哨点医院的腹泻患儿粪便标本1 143例,进行轮状病毒核酸实时荧光定量PCR检测,对检测阳性的样本用巢式反转录PCR法进行G、P分型鉴定,应用SPSS 16.0软件进行统计分析。结果 2015年共收集哨点医院腹泻患儿粪便标本1 143例,其中轮状病毒阳性标本149例,感染率为13.03%,以0月龄~6月龄患儿所占比例最高;发病季节集中在春季、冬季(1月、12月);对其中141例RV阳性进行VP7血清型分型,G9型为优势株,占70%,其次为G1型、G2型、G3型,G3+G9混合型占10%;143例进行VP4基因分型,P[8]型为最流行的型别,占88%。结论轮状病毒是引起深圳地区婴幼儿腹泻的主要病原之一,主要流行株为G9P[8]型,与全国其他地区差异较大。这是深圳地区轮状病毒分型的首次报道。

Objective To investigate the epidemiological characteristics and gene subtypes of rotavirus causing infantile diarrhea in 2015,Shenzhen. Methods 1 143 stool specimens were collected from diarrhea children who were hospitalized in sentinel hospitals in Shenzhen during 2015. Real- time fluorance PCR was performed to identify the nucleic acid of rotavirus,and the positive samples were examined by nested reverse transcription PCR method for typing identification on G,P,and SPSS 16. 0was applied for data analysis. Results 1 143 stool specimens were collected from diarrhea children in sentinel hospitals in2015,of which rotavirus was detected in 149 specimens,with the infection rate of 13. 03%. A peak age distribution of rotavirus was 0- 6 months old; rotavirus infection peaked in spring and winter months（ January and December）. 141 strains with positive RV underwent VP7 serum typing,with G9 as the predominant（ 70%）,followed by G1,G2,G3 and G3 ＋ G9（ 10%）; 143 specimens underwent VP4 genotyping,with P[8]（ 88%） as the dominant. Conclusion Rotavirus is one of the main pathogens causing diarrhea in infants and young children in Shenzhen area. The main epidemic strain is G9P[8],which is different from other regions of the country. This is the first report of rotavirus subtype in Shenzhen.