糖肾宁对自发性2型糖尿病模型小鼠肾纤维化的影响

Effects of Tangshenning on Renal Fibrosis of Spontaneous Type 2 Diabetic KK-Ay Mice

目的:观察糖肾宁对自发性2型糖尿病KK-Ay小鼠肾纤维化的影响。方法:采用自发性2型糖尿病KK-Ay小鼠建立糖尿病肾病模型,按照数字表法随机分为模型组、糖肾宁组和缬沙坦组各20只,设立C57BL/6J小鼠20只为空白组;药物干预12周后,采用免疫组织化学法检测小鼠血小板衍生因子(platelet derived growth factor,PDGF)、血小板衍生生长因子受体(platelet-derived growth factor receptor,PDGFR)、肝细胞生长因子(hepatocyte growth factor,HGF)蛋白表达水平。结果:与空白组比较,模型组PDGF、PDGFR蛋白表达明显升高(P<0.05),HGF蛋白表达明显减少(P<0.05);与模型组比较,缬沙坦组和糖肾宁组PDGF、PDGFR均有不同程度降低(P<0.05),HGF表达明显升高(P<0.05);各给药组间无显著差异(P>0.05)。结论:糖肾宁能够抑制PDGF、PDGFR蛋白的表达,促进HGF蛋白的表达,抑制肾脏纤维化,保护肾功能。

Objective ： To observe the effect of Tangshenning on renal fibrosis of KK-Ay mice with spontaneous type 2 diabetes melli- tus. Methods ： Diabetic nephropathy model was established by spontaneous type 2 diabetes mellitus （KK-Ay） mice. Sixty diabetic C57BL / 6J mice were randomly divided into model group,Tangshenning group and Valsartan group,with 20 mice in each group. Twenty C57BL/6J mice were in blank group. Twelve weeks after drug intervention, immunohistochemical method was used to de- tect the protein expression levels of the platelet-derived growth factor （PDGF）, the platelet-derived growth factor receptor （PDG- FR） and the hepatocyte growth factor （HGF）. Results： Compared with that of the blank group,the increase of the protein expres- sion of PDGF and PDGFR of the model group was significantly higher （ P 〈 0.05 ） and the protein expression of HGF was signifi- cantly lower than that of the blank control group （ P 〈 0.05 ）. Compared with that of the model group, the levels of PDGF and PDGFR of the Tangshenning group and the Valsartan group decreased to certain degrees respectively （ P 〈 0.05 ） and there was a significant increase in the protein expression of HGF（P 〈 0.05 ）. There were no significant differences in the above mentioned in- dexes between each of the administrated groups （ P 〉 0.05 ）. Conclusion ： Tangshenning can inhibit the expression of PDGF and PDGFR protein and promote the expression of HGF protein so as to inhibit the renal fibrosis and protect the renal function.