基于动物物质与能量代谢检测评价3种肾阴虚证模型建立方法

Evaluation of Three Kinds of Kidney Yin Deficiency Modeling Based on Animal Substance and Energy Metabolism Detection

目的:通过对动物物质与能量代谢相关指标的检测,比较3种肾阴虚证动物模型的科学性与可靠性。方法:40只小鼠按照体质量均衡原则分为正常组、氢化可的松组、甲状腺素联合利血平组及热性中药组,每组10只。正常组每天给予生理盐水灌胃,氢化可的松组隔日皮下注射氢化可的松50 mg·kg^(-1),甲状腺素联合利血平组灌胃甲状腺素150mg·kg-1加肌肉注射利血平1 mg·kg^(-1),热性中药组灌胃热性中药煎煮液按生药量为15 g·kg^(-1),建立3种肾阴虚证模型小鼠。分别观察动物采食量、饮水量、体质量、自主活动情况、呼吸耗氧量、体温、体能耐力、血清环磷酸腺苷(cyclic adenosine monophosphate,cAMP)与环磷酸鸟苷(cyclic guanosine monophosphate,cGMP)及其比值等指标,考察3种肾阴虚证模型小鼠体质及自身能量代谢的差异。结果:造模后3种肾阴虚证模型小鼠饮水量与饮食量均增加,体质量均降低。此外,氢化可的松组小鼠呼吸耗氧量降低、四肢体温及肛温明显升高,cAMP/cGMP比值明显升高,与正常组比较,有显著性差异(P<0.05)。自主活动、体能耐力及肾脏系数变化不明显,与正常组比较,无显著性差异;甲状腺素联合利血平组小鼠肾脏系数、单位时间耗氧量与正常组比较,明显降低,自主活动及体能耐力及有降低趋势,但与正常组比较,无显著性差异。四肢体温、肛温及血清cAMP与cGMP水平明显升高,与正常组比较,有显著性差异(P<0.05);热性中药组小鼠单位时间耗氧量明显降低,自主活动次数增加,与正常组比较,有显著性差异,肾脏系数、四肢体温、肛温、体能耐力及血清cAMP与cGMP水平及其比值变化均不明显,与正常组比较,无显著性差异。结论:皮下注射氢化可的松、灌胃甲状腺素联合肌肉注射利血平混悬液可在短时间内建立肾阴虚证模型小鼠,灌胃热性中药在短时间内无法成功建立肾阴虚证模型小鼠。

Objective：To examine the scientific nature the animal Kidney Yin Deficiency modeling by comparing three indicators of animal models in substance and energy metabolism. Methods：Forty mice were randomly divided into normal group, Hydroeortisone group, Thyroxine combined with Reserpine group and Heat-natured traditional Chinese medicine group according to the principles of body mass balance. In the normal group, normal saline was given orally every day. Hydrocortisone group was injected hydrocorti- sone 50 mg·kg-1 every other day,and Thyroxine combined with Reserpine group was given gavage of Thyroxine 150 mg· kg-1and intramuscular injection of Reserpine 1 mg ~ kg-1, respectively. Heat-natured traditional Chinese medicine group was treated with traditional Chinese medicine decoction with amount of 15 g· kg-1 crude drug. Three kinds of mice models with Kidney Yin Deficiency syndrome were established in this way. The food intake, body weight, body weight, spontaneous activity, oxygen con- sumption, body temperature, physical endurance, cyclic adenosine monophosphate （cAMP） and cyclic guanosine monophosphate （cGMP） and the ratio of the two were examined to find out the differences in rats＇ constitution and their energy metabolism. Resuits：The water consumption and food intake of the three Kidney Yin Deficiency Syndrome models were increased and the body weight was decreased. In addition, the oxygen consumption decreased yet the limbs temperature and rectal temperature increased obviously in Hydrocortisone group, with the ratio of cAMP / cGMP being significantly increased, which was significantly different from that of the normal group. However, there was no significant difference in spontaneous activity, physical endurance and kidney coefficient between the two groups. Compared with the normal group, the renal coefficient and unit time oxygen consumption of Thyroxine combined with Reserpine group were significantly lower than those of the normal group yet there was no significant difference compared with that of the normal group. The body temperature, rectal temperature, serum cAMP and cGMP levels of the four extremities were significantly higher than those of the normal group, and the oxygen consumption per unit time in the Heat-na- tured Chinese medicine group was significantly lower than that of the normal group There were no significant differences in renal function, body temperature, rectal temperature, body endurance, serum cAMP and cGMP levels and ratio between the two compared with the normal group. Conclusion：Mice model with Kidney Yin Deficiency Syndrom can be established in a short time yet it can- not be successfully established in a short time with Hypodermic injection of Hydrocortisone, intragastric administration of Thyroxine combined with intramuscular injection of Reserpine suspension and Heat-natured traditional Chinese medicine.