食管癌血红素加氧酶-1表达水平与氟尿嘧啶疗效的关系研究

Study on HO-1 expression in hunman esophageal carcinoma and correlates with chemotherapy sensitivity of fluorouracil

目的本实验旨在探讨Eca109食管鳞癌细胞中血红素加氧酶-1(HO-1)的表达水平与5氟尿嘧啶(5-FU)化疗敏感性的关系。方法培养Eca109食管鳞癌细胞,应用RT-PCR及Western blot方法分别检测不同浓度(0、20、80、100μmol/L)ZnppⅨ处理后细胞HO-1基因及HO-1蛋白表达水平,应用噻唑蓝(MTT)法检测各实验组细胞生长曲线及药物抑制率。结果随着培养基ZnppⅨ浓度的增加,细胞抑制率显著增高,80μmol/L ZnppⅨ组显著高于20μmol/L ZnppⅨ组(P<0.05)。各组间HO-1mRNA的表达量依次为0.50±0.17、0.55±0.15、0.58±0.09、0.55±0.16,组间比较差异无统计学意义(P>0.05)。各组间HO-1的表达量依次为0.85±0.07、0.63±0.11、0.43±0.12、0.25±0.10,组间差异有统计学意义(F=20.01,P<0.01)。结论Eca109食管癌细胞抑制率与ZnppⅨ浓度正相关,而ZnppⅨ不是从基因水平,而是从蛋白的表达水平抑制HO-1的表达。

Objective To explore the HO-1expression levels with 5-FU chemosensitivity in esophageal squamous cell carcinoma.Methods Eca109 cells used in all experiments,MTT assay was used in cell growth curve and inhibit rate.RT-PCR and Western blot was used to detect HO-1in cells treated with different concentrations of ZnppⅨ（0,20,80,100μmol/L）.Results The inhibitory rate of cells was significantly increased when the concentrition of ZnppⅨ increased.The inhibitory rate of cells in 80μmol/L ZnppⅨ was higher than 20μmol/L ZnppⅨ group（P〈0.05）.The expression of HO-1mRNA in each group was 0.50±0.17,0.55±0.15,0.58±0.09 and 0.55±0.16,respectively,there was no significant difference between the two groups（P〉0.05）.The expression of HO-1was 0.85±0.07,0.63±0.11,0.43±0.12 and 0.25±0.10,respectively.The expression of HO-1had significant difference（F=20.01,P〈0.01）.Conclusion Eca109 cells inhibition rate positive correlated with ZnppⅨ concentrations,and ZnppⅨ were inhibited the expression of HO-1.not from gene level,but after the translation level.