聚合酶链反应和反向斑点杂交技术检测人乳头瘤病毒感染

Application value of PCR and RDB in detecting HPV infection

目的:研究聚合酶链反应(polymerase chain reaction,PCR)和反向斑点杂交(reverse dot blot,RDB)技术在人乳头瘤病毒(human papillomavirus,HPV)感染检测中的情况,进一步研究其应用价值。方法:收集2013年3月至2015年6月宫颈癌、尖锐湿疣、宫颈上皮内瘤变三种类型疾病共320例脱落细胞标本,采用PCR和RDB杂交技术对HPV基因型进行检测,分析检测数据。结果:在宫颈癌、尖锐湿疣、宫颈上皮内瘤变的感染中HPV单基因型感染均多于多重基因型感染,但不同疾病中单基因型感染患者比率差别不明显,分别为82.50%、84.06%、84.30%;不同疾病中多重基因型感染患者例数分别为17.50%、15.94%、15.70%,两种类型感染情况比较,差异无统计学意义(P>0.05);320例标本中检测出的210例阳性标本进行分型,反复检验后数据显示HPV16为最主要的感染基因型。结论:PCR和RDB技术是HPV基因型检测的有效方法,也是关于进行HPV感染防治的有效检测指标。

Objective：To study the value of polymerase chain reaction（ PCR） and reverse dot blot（ RDB） in the detection of human papillomavirus（ HPV） infection. Methods：320 exfoliative cells samples of cervical cancer,condyloma acuminatum,and cervical intraepithelial neoplasia from March 2013 to June 2015 were collected and the HPV genotype was detected by PCR and RDB. Results：The HPV haplotype infection rates in cervical cancer,condyloma acuminatum,and cervical intraepithelial neoplasia were higher than the infection rates in multiple genotypes. The HPV haplotype infection rates were 82. 50 %,84. 06 % and 84. 30 % respectively and in multiple genotypes were 17. 50 %,15. 94 % and15. 70 % respectively. There was no significant difference of infection in each group（ P〈0. 05）. HPV16 was the most common infection genotype in the 210 positive samples after repeated detection. Conclusion：PCR and RDP are effective methods in the detection of HPV genotype as well as in HPV prevention and treatment.