微小RNA-373对乳腺癌细胞增殖和侵袭能力的影响及其机制

Effects of microRNA-373 on the proliferation and invasiveness of breast carcinoma and its mechanisms

目的 探讨微小RNA （miR）-373对乳腺癌细胞增殖和侵袭能力的影响及其机制.方法 80例乳腺癌患者癌组织及其对应的癌旁组织均取自于2010至2014年在郑州大学第一附属医院手术切取的标本.通过实时荧光定量-聚合酶链反应（RT-PCR）检测miR-373在乳腺癌患者癌组织及其对应的癌旁组织中的表达.细胞实验分3组：正常对照组、miR-373类似物组和miR-373抑制剂组.利用CCK-8实验、Transwell体外侵袭实验检测miR.373表达对乳腺癌细胞增殖、侵袭及迁移能力的影响.利用流式细胞仪检测miR-373表达对细胞凋亡的影响.结果 乳腺癌组织中miR-373的表达显著低于癌旁正常乳腺组织（0.21±0.09比0.98±0.23）（P ＜0.001）;miR-373能抑制B细胞淋巴瘤因子（Bcl）-6表达.miR-373类似物组细胞凋亡率显著高于正常对照组（18.29％±0.29％比4.91％±0.31％）,细胞增殖率、侵袭能力、迁移能力均显著低于正常对照组[（0.69±0.11）比（1.25±0.12）（第5天）、（30.50±0.20）比（66.50±0.42）、（29.50±0.21）比（48.50±0.31）,均P＜0.05].结论 miR-373表达对乳腺癌细胞的增殖和侵袭能力有抑制作用,其机制可能是miR-373低表达促进Bcl-6上调从而促进乳腺癌细胞增殖增加其侵袭作用.

Objective To explore the effects of microRNA （miR）-373 on proliferation and invasion of breast cancer cells and its mechanisms.Methods Breast carcinoma tissues and its corresponding normal tissues （80 cases） were obtained from the First Affiliated Hospital of Zhengzhou University from 2010 to 2014.Quantitative real-time （RT）-PCR was used to detect the expression of miR-373 of breast carcinoma tissues and its corresponding normal tissues.The cellula experiment was divided into three groups：the nomal control group,the miR-373 mimic group and the miR-373 inhibitor group.The proliferation was detected by Cell Counting Kit.8 （CCK-8） method and the invasion and migration were detected by Transwell method.Effect of miR-373 on cell apoptosis was determined by Flow Cytometry.Results Expression of miR-373 in breast cancer tissue was significant lower than the normal breast tissues （0.21 ±0.09 vs 0.98 ±0.23,P 〈 0.001）;expression of miR-373 contributed to the inhibition of Bcl-6 expression.The apoptosis rate in miR-373 was higher than normal control group （18.29% ± 0.29% vs 4.91% ± 0.31%）,cell proliferation,cell invasion and cell migration were lower than normal control group [（0.69 ± 0.11） vs （1.25±0.12） （the fifth day）,（30.50 ±0.20） vs （66.50 ±0.42）,（29.50 ±0.21） vs （48.50 ± 0.31）,all P 〈 0.05].Conclusion miR-373 plays a suppressive role in proliferation and invasion of breast carcinoma,and the downregulation of miR-373 promotes the invasion of breast cancer which may mediate by the increased expression of Bcl-6.