摘要
目的采用高效液相色谱法(HPLC)同时测定宽筋藤药材中盐酸药根碱、盐酸假巴马汀和盐酸巴马汀三种成分的含量。方法使用OMNI C18色谱柱(4.6 mm×250 mm,5μm);流动相为乙腈-2.5 mmo L/L庚烷磺酸钠和50 mmo L/L磷酸二氢钾(含三乙胺0.14%)(22:78);检测波长345 nm;柱温40℃;流速1.0 m L/min。结果盐酸药根碱、盐酸假巴马汀和盐酸巴马汀分别在1.872~56.16μg/m L、8.37~167.4μg/m L、2.014~60.42μg/m L浓度范围内具有良好的线性;平均加样回收率依次为:102.19%(RSD=1.70%)、102.89%(RSD=1.09%)、102.38%(RSD=1.17%)。结论建立的分析方法准确、可靠,重复性好,可用于宽筋藤药材的质量控制。
Objective A high performance liquid chromatography (HPLC) method was developed to determine ja- trorrhizine, pseudopalmantine and palmatine chloride in Tinospora sinensis. Methods The chromatographic separation was performed on OMNI C18 column (4.6 mm × 250 mm, 5 p,m) with the gradient elution by acetonitrile -2.5 mmoL/ L sodium heptane and 50 mmoL / L potassium dihydrogen phosphate (containing O. 14 % triethylamine) (22:78 ) at a flow rate of 1.0 mL / min. The detection wavelength was set at 345 nm, column temperature was maintained at 40 ℃. Results The linear ranges of jatrorrhizine, pseudopalmantine and palmatine chloride were 1. 872 -56.16 μg/mL,8.37 × 167.4 μg/mL,2.014 ~60.42 μg/mL respectively. The average recovery were 102.07 % (RSD=0. 02 % ) ; 99.91%( RSD =0.03 % ) ; 99.11% ( RSD=0.02 % ), respectively. Conclusion The established method is accurate, reliable, reproducible and can be used for the quality control of Tinospora sinensis.
出处
《现代中医药》
CAS
2017年第1期74-78,共5页
Modern Chinese Medicine
关键词
宽筋藤
HPLC
盐酸药根碱
盐酸假巴马汀
盐酸巴马汀
含量测定
Tirvaspora sinensis
HPLC
jatrorrhizine
pseudopalmantine
palmatine chloride
quantitative determi- nation