长链非编码RNALINC00261在人胃癌组织的表达及其临床意义

Clinical significance and expression of long chain noncoding RNA LINC00261 in human gastric carcinoma

目的探讨长链非编码RNA LINC00261在人胃癌组织中的表达及临床意义。 方法收集72对人胃癌和配对正常胃黏膜组织，采用实时荧光定量聚合酶链反应（FQ-PCR）方法检测组织中LINC00261表达水平，分析其表达与患者性别、年龄、肿瘤部位、肿瘤直径、组织分化程度、肿瘤浸润深度、淋巴结转移、远处转移、TNM分期、脉管内有无癌栓及5年存活率等临床病理特征的相关性。 结果与配对正常组织比较，LINC00261呈低表达。在72例胃癌组织中LINC00261低表达率为76.38%（55/72），而正常胃组织LINC00261低表达率为2.78%（2/72），两者差异有统计学意义（χ2＝81.568，P＝0.000）。LINC00261低表达与患者性别（χ2＝0.066，P＝0.936）、年龄（χ2＝0.000，P＝0.988）、肿瘤位置（χ2＝1.332，P＝0.722）、肿瘤直径（χ2＝2.037，P＝0.154）、组织分化程度（χ2＝0.701，P＝0.704）及远处转移（χ2＝0.003，P＝0.955）均无明显相关，但与肿瘤浸润深度（χ2＝9.519，P＝0.023）、淋巴结转移（χ2＝9.577，P＝0.023）、TNM分期（χ2＝13.299，P＝0.004）、脉管内有无癌栓（χ2＝5.318，P＝0.021）及5年存活率（χ2＝6.237，P＝0.013）均显著相关。 结论LINC00261可能是胃癌基因诊断和治疗中重要靶点之一。

Objective To investigate the clinical significance of long chain noncoding RNA LINC00261 in human gastric carcinoma. Methods 72 pairs of human gastric cancer and matched normal gastric tissue were collected, and LINC00261 were detected by real - time fluorescent quantitative polymer- ase chain reaction （FQ- PCR）. The relation of between LINC00261 expression and patient＇s sex, age, tumor location, tumor diameter, histological differentiation, depth of tumor invasion, lymph node transfer shift, distant metastasis, TNM staging, vascular tumor thrombus and five -year survival rate was analyzed, respectively. Results Compared with matched normal tissues, LINC00261 is low - expression in gastric cancer. The expression rate of LINC00261 in 72 pairs of gastric carcinoma was 76. 38% （55/72）, while the expression rate of LINC00261 in normal gastric tissues was 2. 78% （2/72）. The difference is very sig- nificant （X： = 81. 568, P = 0. 000）. The results showed that LINC00261 expression was not significantly correlated with the patient gender （χ2 = 0. 066, P = 0. 936）, age （χ2 = 0. 000, P = 0. 988 ）, tumor loca- tion （χ2 = 1. 332, P = 0. 722 ）, tumor diameter （χ2 = 2. 037, P = 0. 154 ）, histological differentiation （χ2 =0. 701, P =0. 704）, and distant metastasis （χ2 =0. 003, P =0. 955） had no significant correlation, but was significantly correlated with tumor invasion depth （χ2 = 9. 519, P = 0. 023 ） , lymph node metasta- sis （χ2 =9. 577, P=0. 023）, TNM staging （χ2= 13. 299, P=0. 004） , tumor thrombus （χ2 =5. 318, P = 0. 021 ）, and five - year survival rate （χ2 = 6. 237, P = 0. 013 ）, respectively. Conclusion LINC00261 might be one of the important targets in the diagnosis and treatment of gastric cancer.