核磁共振波谱法研究聚山梨酯80结构

NMR method for the structure of polysorbate 80

目的:建立采用核磁共振波谱法研究聚山梨酯80的结构。方法:核磁共振波谱是在装配5 mm BBO探头的Bruker Ascend-500核磁共振谱仪上采集,测量温度25℃。一维氢谱弛豫时间2s,谱宽δ_H 15,采样点数64 k,扫描次数128次;一维碳谱弛豫时间1 s,谱宽δ_C 236.6,采样点数64 k,扫描次数32 768次;二维谱图弛豫时间1.4 s,扫描次数16次。结果:核磁共振氢谱(~1H谱)、碳谱(^(13)C谱)、二维异核单量子相关谱(二维HSQC谱)、异核多量子相关谱(HMBC谱)、同核位移相关谱(COSY谱)可以解析出聚山梨酯80的结构。结论:核磁共振波谱法操作简单,结果明确,专属性强,是研究聚山梨酯80结构较好的方法。

Objective：To establish a method for studying the structure of polysorbate 80.Methods：Nuclear magnetic resonance（NMR） spectra were collected at Bruker Ascend 500 spectrometer with 5 mm BBO probe at 25 ℃.In 1D ~1H-NMR spectra,the relaxation delay（D1） was 2 s,spectral width（SW） was δ_H 15,data point was 64 k and number of scan was 128.In 1D ^（13）C-NMR spectra,the D1 was 1 s,SW is was δ_c 236.6,data point was 64 k and number of scan was 32 768.In 2D NMR spectra,the D1 was 1.4 s and the number of scan was 16.Results：The structure of polysorbate 80 was characterized by ~1H-NMR,^（13）C-NMR,HSQC-NMR,HMBC-NMR,COSY-NMR spectra Conclusion：NMR spectra are specific,simple and convenient method for studying the structure of polysorbate 80.