补肾活血化痰对血管性痴呆小鼠GSH-Px表达的影响

Effect of Tonifying Kidney,Promoting Blood Circulation and Reducing Phlegm Method on GSH-Px Expression for Vascular Dementia Mice

目的:研究脑力苏胶囊对血管性痴呆(VD)小鼠一般情况和GSH-Px表达的影响,探讨该方药对大脑的部份保护作用及其防治机制。方法:采用反复夹闭颈总动脉方法建立血管性痴呆小鼠模型。将造模成功后的小鼠随机分成5组:假手术组、模型对照组、西药治疗组、脑力苏胶囊高剂量组和脑力苏胶囊低剂量组。造模成功后,给药第11天观察小鼠一般情况变化,第14天后快速断头取脑,制成10%的组织匀浆液,4℃下离心,取血浆测定谷胱甘肽过氧化物酶(GHS-Px)活性的变化。结果:实验结果显示造模后的VD小鼠体重增长速度均有不同程度的降低,各治疗组小鼠的体重增加明显;治疗组GSH-PX水平与模型组比较均出现不同程度的升高,以脑力苏各组表现明显(P<0.01)。结论:脑力苏胶囊可能通过提高脑组织中GHS-Px活性,从而提高血管性痴呆小鼠的学习记忆能力。

Objective： To study Naolisu Capsule on vascular dementia （VD） mice, and to explore the prescription on the brain protective effect and its control mechanisms. Methods ： The method of repeated occlusion of common carotid artery to estab- lish a mouse model of vascular dementia. After the success of the modeling of the mice were randomly divided into five groups ： sham operation group; model controlled group; western medicine treatment group; Naolisu Capsule high dose group and Naolisu Capsule capsule low dose group. General condition would be observed after successful modeling on the 11th days of mice adminis- tered, rapid decapitation, brain was removed tissue made of 10% of the liquid, 4℃, under centrifugation, taking plasma gluta- thione peroxide HRP （GHS-Px） activity changes on the 14th day. Results： The results showed that the VD growth rate of body weight of mice were reduced to varying degrees after modeling, all treated groups of mice gained weight significantly; GSH-PX levels of the treatment group were compared with the model group, especially the different groups of Naolisu Capsule （P 〈 0. 01 ）. Conclusion ： The Naolisu Capsule can enhance the brain tissue through the GHS-Px activity, thereby enhancing vascular de- mentia learning and memory in mice.