贵州省部分地区H7亚型禽流感监测与分析

Detection and Analysis of H7 Subtype Avian Influenza in 2015 in Guizhou Province

为了解贵州省H7亚型禽流感感染情况,采用HI试验对全省9个地区家禽血清样本进行血清抗体检测,PT-PCR方法对家禽咽-肛拭子、养禽场环境样本和野鸟粪便进行病原核酸检测。结果显示:在1570份家禽血清样本中,H7亚型禽流感血清抗体阳性率为0.45%(7/1570);在350份家禽咽-肛拭子和310份养殖场环境样本中,均未检测出H7亚型禽流感病毒核酸;在160份野鸟粪便中,有1份为H7亚型禽流感病毒核酸阳性,阳性率为0.63%(1/160)。试验提示,在贵州省养禽场中不存在H7亚型禽流感感染,但迁徙野禽中可能存在H7亚型禽流感隐性感染,应当引起重视。

To master the infection situation of H7 subtype avian influenza （AI - H7 ） in Guizhou province the serum samples of poultry were collected to detect the AI - H7 antibodies by HI test, and the samples o f swallow - anal swabs and environment and wild bird faeces were gathered to determine the pathogenic nucleic acid of AI - H7 by RT - PCR method. The results showed that the positive rate of AI - H7 antibodies was 0.45% in 1570 of poultry serum samples. And the pathogenic nucleic acid of AI - H7 were not detected in 350 of swallow-anal swabs and 310 of farm environment samples, but 1 sample in 160 of wild bird faeces which the positive rate was 0.63%. These results suggested that there were no infection of AI - H7 in poultry farm, but maybe latent infection in wild birds and should be paid attention.