有机硅季铵盐浸泡纯钛对白假丝酵母菌生长的抑制作用

The inhibition of the organosilicon quaternary ammonium salt treated pure titanium to the Candida albicans

目的通过有机硅季铵盐溶液浸泡处理纯钛试件,研究该方法对白假丝酵母菌的抗菌效果。方法制作纯钛试件,将其随机分为A^F的6组,A^E组分别用浓度为1%、5%、10%、30%、60%的有机硅季铵盐溶液对纯钛试件进行抗菌处理,F组用蒸馏水浸泡处理作为对照,每组再分4小组,各小组处理时间分别为30、60、120、240 min,用比浊法统计分析有机硅处理后的纯钛试件对白假丝酵母菌生长的影响。将试件分别进行0、2 500、5 000、10 000次的冷热循环实验后再进行细菌培养,检验有机硅季铵盐是否具有长效抗菌性。结果不同处理浓度对白假丝酵母菌生长有影响;不同处理时间对白假丝酵母菌生长无影响;处理浓度和处理时间两因素之间存在交互作用。1%的有机硅季铵盐浸泡纯钛即可使其产生抗菌性,浸泡120 min抗菌效果最佳。冷热循环5 000次后纯钛仍具有抗菌性;10 000次后无抗菌性。结论有机硅季铵盐溶液浸泡纯钛,对白假丝酵母菌生长具有抑制作用,可使纯钛具有长效抗菌性能。

Objective This study investigated the in vitro antimicrobial ability of pure titanium when pre-soaked with organosilicon quaternary ammonium salt. Methods The pure titanium specimens were randomly divided into six groups, five of which were soaked with different concentrations of organic silicon quaternary ammonium salt solution at 1%, 5%, 10%, 30%, and 60%, while one was treated with distilled water as a control. Each group was further divided into four different treatment times（30, 60, 120, and 240 min）. The turbidimetric method was used to analyze the effect of organic silicon to the growth of Candida albicans on the pure titanium. The experimental groups were subjected to 0, 2 500, 5 000, and 10 000 cycles of hot and cold experiments and exposure to bacterial culture. The long-term antimicrobial properties of the treated titanium was calculated through a hot and cold cycle experiment. Results Different concentrations significantly affect the growth of Candida albicans, but the processing time does not. There is an interaction between concentration and processing time. Titanium soaked with 1% organosilicon quaternary ammonium salt is potentially antibacterial, and its antibacterial effect reaches a maximum upon 120 min soaking. After 5 000 cycles hot and cold experiments, the pure titanium retained its antibacterial property; however, the antibacterial activity was lost after 10 000 cycles. Conclusion After soaking with the organosilicon quaternary ammonium salt solution, the pure titanium can maintain its long- acting antibacterial properties .