胰岛素对糖尿病大鼠种植体周骨组织代谢影响的研究

Effect of insulin on bone metabolism around implant in diabetic rats

目的探索胰岛素治疗对于高糖环境下种植体周骨组织代谢及种植体-骨结合的影响。方法构建糖尿病大鼠模型,在大鼠股骨植入钛种植体,并给予胰岛素治疗;同时通过成骨细胞培养,体外构建高糖环境,给予胰岛素干预。结果糖尿病大鼠种植体周骨组织形成和矿化明显降低,胰岛素干预治疗能起到明显的改善作用;此外,胰岛素干预后成骨细胞中胰岛素受体表达明显增加。结论胰岛素也许通过胰岛素信号通路调控高糖环境中成骨细胞功能,改善骨组织的形成和矿化。

Objective We studied the bone metabolism and implant bone contact in diabetic subjects treated with insulin. Methods Diabetic rat models were established and implanted with titanium implants and insulin treatment. The osteoblasts were cultured in high glucose amounts and treated with insulin. Results Diabetes decreased bone formation and mineralization around the rat implants, which were improved by insulin treatment. However, the implant-bone contact in insulin-treated diabetic rats remained less than those in normal rats. Moreover, insulin treatment increased the insulin receptor expression in osteoblasts that have been cultured in high glucose amounts. Conclusion Our results indicated the role of insulin in osteoblast function in diabetic rats probably through insulin signaling; moreover, insulin mediates bone metabolism and improves bone formation and mineralization in hyperglycemic conditions.