布拉氏酵母菌对大鼠肠道菌群及血清炎性因子的干预研究

A Study on Saccharomyces Boulardii in Intervention of Intestinal Flora and Inflammatory Factors of Rats

目的观察高脂饮食对大鼠肠道菌群及血清炎性因子的影响,探讨布拉氏酵母菌散剂在调节肠道菌群中的作用。方法将36只大鼠随机分为A、B、C组,每组12只。A组喂养普通饲料,B组喂养高脂饲料,C组喂养高脂饲料,并予布拉氏酵母菌干预。实验初始、第10周末检测血清脂多糖(LPS)、白细胞介素-6(IL-6)、肿瘤坏死因子-α(TNF-α)及粪便中双歧杆菌、乳酸杆菌、拟杆菌、大肠杆菌、肠球菌数量。结果 3组初始时LPS、TNF-α、IL-6差异无统计学意义(P>0.05);10周末时B、C组LPS、TNF-α较初始时明显升高(P<0.05、0.01),A组无改变(P>0.05);B组IL-6较初始时升高(P<0.01),A、C组无改变(P>0.05);10周末时B组LPS、TNF-α、IL-6均高于A、C组(P<0.01),C组TNF-α高于A组(P<0.01)。3组初始乳酸杆菌、双歧杆菌、肠球菌、拟杆菌、大肠杆菌比较差异无统计学意义(P>0.05);10周末时B、C组乳酸杆菌、双歧杆菌、肠球菌较初始时升高(P<0.01),A组无改变(P>0.05),B组低于A组及C组(P<0.01),C组低于A组(P<0.05);10周末时B组拟杆菌较初始时降低(P<0.01),A、C组无改变(P>0.05),B组低于A、C组(P<0.01);10周末时B组大肠杆菌较初始时升高(P<0.01),A组无改变(P>0.05),C组降低(P<0.05),B组高于A、C组(P<0.01),A组高于C组(P<0.05)。结论高脂饮食喂养会增加大鼠血清炎性因子释放,改变肠道菌群结构;布拉氏酵母菌可稳定肠道菌群结构,减少炎性因子释放。

Objective To observe influence of high-fat diet on intestinal flora and inflammatory factors of rats,and to investigate effect of Saccharomyces Boulardii powders in regulation of intestinal flora. Methods A total of 36 rats were randomly divided into group A,B and C（ n = 12 for each group）. Group A was given normal diet. Group B was given high-fat diet. Group C was given high-fat diet and Saccharomyces boulardii powder. Lipopolysaccharide（ LPS）,interleukin-6（ IL-6） and tumor necrosis factor-α（ TNF-α） in serum and numbers of bacillus bifidus,lactobacilli,bacteroides,escherichia coli and enterococcus in feces at the beginning and at the end of 10^ thweeks of experiment were detected. Results There were no significant differences in LPS,TNF-α and IL-6 levels at the beginning experiment among 3groups（ P〉0. 05）. At the end of 10 ^thweeks of experiment,LPS and TNF-α levels in group B and C were significantly increased（ P〈0. 05,P〈0. 01）,while the levels showed no changes in group A（ P〉0. 05）; IL-6 level in group B was significantly increased（ P〈0. 01）,while the levels showed no changes in group A and C compared with those at the beginning experiment（ P〈0. 05）. Levels of LPS,TNF-α and IL-6 in group B were significantly higher than those in group A and C（ P〈0. 01）,TNF-α level in group C was significantly higher than that in group A at the end of 10^ thweeks of experiment（ P〈0. 01）. There were no significant differences in numbers of fecal bacillus bifidus,lactobacilli,bacteroides,escherichia coli and enterococcus at the beginning experiment among 3 groups（ P〈0. 05）. At the end of 10 th weeks of experiment,numbers of fecal lactobacilli,bacillus bifidus and escherichia coli in group B and C were significantly higher（ P〈0. 01）,while the numbers showed no changes in group A compared with those at the beginning experiment（ P〉0. 05）; the numbers in group B were significantly lower than those in group A and C（ P〈0. 01）,and the numbers in group C were lower than those in group A（ P〈0. 05）. At the end of 10 thweeks of experiment,number of fecal bacteroides in group B was lower（ P〈0. 01）,while the numbers showed no changes in group A and C compared with those at the beginning experiment（ P〈0. 05）,and the number in group B was significantly lower than those in group A and C（ P〈0. 01）; the number of fecal escherichia coli in group B was significantly higher（ P〈0. 01）,while the number in group A showed no change（ P〉0. 05）,but the number in group C was significantly lower compared with those at the beginning experiment（ P〈0. 05）; the number of fecal escherichia coli in group B was higher than those in group A and C（ P〈0. 01）,and the number in group A was higher than that in group C（ P〈0. 05）. Conclusion High-fat diet may increase release of inflammatory factors and change intestinal flora of rats. Saccharomyces boulardii can stabilize intestinal flora and reduce release of inflammatory factors.