不同产羔数绵羊性腺轴比较转录组研究

Comparative Transcriptome Research of Gonadal Axis for Different Lambing Number of Sheep

试验通过对巴美肉羊性腺轴进行转录组分析,旨在挖掘影响多羔性状的关键功能基因。分别选取双羔组巴美肉羊5只、单羔组4只,利用转录组测序(RNA-Seq)技术对下丘脑、垂体、卵巢转录组文库进行测序,然后对得到的测序数据进行生物信息学分析。测序数据经过质量控制后,27个样品共得到112Gb的有效数据。差异基因分析表明,与单羔组相比,双羔组下丘脑中,上调表达基因111个,下调表达基因106个;垂体组织中,上调表达基因97个,下调表达基因142个;卵巢组织中,上调表达基因182个,下调表达基因67个。功能富集性分析表明,神经活性的配体-受体相互作用信号通路在下丘脑-垂体-卵巢性腺轴调控排卵及卵泡发育方面发挥着重要的调控作用。通过不同产羔数巴美肉羊性腺轴比较转录组研究,提供了全部的转录本信息,筛选了影响产羔数的相关功能基因,丰富和补充了绵羊基因组信息,为进一步阐明绵羊繁殖力差异的分子机制奠定基础。

In order to obtain the important functional genes related to prolificacy,transcriptome of gonadal axis 5Bamei mutton with high repeoduction and 4Bamei mutton with low reproduction were analyzed using RNA-Seq technology.The sequencing data was analyzed by bioinformatics methods.After quality control,112 Gb clean data were obtained from 27 libraries of hypothalamus,pituitary and ovary.The differential expression analysis underlined 111 genes highly expressed in the hypothalamus,97 in the pituitary and 182 in the ovary between twinning group and single lambs;106genes lower expression in the hypothalamus,142 in the pituitary and 67 in the ovary between twinning group and single lambs.Functional enrichment analysis revealed thatneuroactive ligand-receptor interaction signaling pathway played an important role in the hypothalamic-pituitary-ovarian axis regulation of ovulation and follicular development.Comparative transcriptomic studies for different lambing number Bamei mutton sheep gonadal axis prodeced all transcript information,provided a list of functiona genes for influencing litter size,enriched and supplemented the sheep genome information,laid the theoretical basis for further elucidation the molecular mechanism of sheep fecundity differences.