结直肠癌及癌旁组织中miR-338-3p及MACC1的表达变化及意义

Expressions and significance of mi R-338-3p and MACC1 in colorectal carcinoma and adjacent non-tumorous tissues

目的观察结直肠癌组织中mi R-338-3p与结直肠癌转移相关基因1(metastasis-associated in colon cancer-1,MACC1)的表达变化,并探讨其意义。方法收集昆明医科大学第二附属医院2015年1月-2015年5月外科手术切除并经病理证实的15例结直肠癌患者,手术时留取癌组织及癌旁组织,采用实时荧光定量PCR检测mi R-338-3p的表达,免疫组化检测MACC1的表达,双荧光素酶报告检测mi R-338-3p与MACC1。结果结直肠癌组织及癌旁组织中mi R-338-3p的相对表达量分别为0.022±0.005和0.071±0.002,差异有统计学意义(P<0.05);MACC1在结直肠癌组织中的阳性表达率(66.7%)显著高于癌旁组织(20.0%),差异有统计学意义(P<0.01);mi R-338-3p与MACC1的表达呈负相关(r=-0.798,P<0.01),mi R-338-3p抑制MACC1的表达。结论mi R-338-3p可能通过转录后基因沉默机制抑制MACCI的表达,从而抑制结直肠癌的发生。

Objective To observe the expressions of mi R-338-3p and MACC1 in colorectal carcinoma tissues and to investigate the significance. Methods The colorectal carcinoma tissues and adjacent non-tumorous tissues were collected（15 patients） respectively in the Second Affiliated Hospital of Kunming Medical University from Jan. 2015 to May.2015,which were confirmed by pathology. Mi R-338-3p was detected by Real-time quantitative PCR. MACC1 was detected by immunohistochemical technique. The correlation between mi R-338-3p and MACC1 was detected by pual luciferase report system. Results The expressions of mi R-338-3p in colorectal carcinoma tissues and adjacent non-tumorous tissues were 0. 022 ± 0. 005 and 0. 071 ± 0. 002,respectively,the difference was significant（ P〈0. 05）; the positive rate of MACC1 in colorectal carcinoma（66. 7%） was higher than that in adjacent non-tumorous tissues（20. 0%）（ P〈0. 01）; there was negative correlation between mi R-338-3p and MACC1（ r =- 0. 798,P〈0. 01）. Mi R-338-3p inhibited the expression of MACC1. Conclusion Mi R-338-3p may inhibit the MACC1 expression by post-transcriptional gene silencing to interdict the occurrence and progress of colorectal carcinoma.