慢性细菌性前列腺炎患者前列腺液中性粒细胞CD64表达的变化

Changes of CD64 expression in succus in patients with chronic bacterial prostatitis

目的观察不同感染程度的慢性前列腺炎患者前列腺液中性粒细胞CD64表达的水平，评价CD64对慢性细菌性前列腺炎的临床诊治价值。方法将100例研究对象分别按照美国国立卫生研究院（NIH）创立的慢性前列腺炎症状量表（chronic prostatitis symptom index, CPSI）分为细菌感染组CPSI高分组和CPSI低分组、非细菌感染组及30例健康对照组，分别应用流式细胞仪检测各组前列腺液中性粒细胞CD64表达，前列腺液常规镜检（EPS-RT）。结果重症组（高分组）CD64表达量为（4857.25±278.45）分子数/细胞，明显高于轻症组（4124.59±197.43），差异具有统计学意义（低分组）（P〈0.05）,轻症组与非细菌感染组、健康对照组比较，差异具有统计学意义（P〈0.01）。非细菌感染组与健康对照组比较，差异无统计学意义。细菌感染组CD64表达与部分白细胞呈正相关，但整体不相关，据受试者数据绘制ROC曲线，当CD64为2135.2分子数/细胞，敏感性为92.9%，特异度为91.7%。 结论定量检测前列腺液中CD64可作为诊断慢性细菌性前列腺炎的敏感指标，具有临床价值。

ObjectiveTo observe the levels of neutrophil CD64 expression in prostatic fluid of patients with chronic prostatitis and different degrees of infection, to evaluate the value of CD64 in diagnosis and treatment of chronic bacterial prostatitis.Methods 100 subjects were divided into two groups according to chronic prostatitis symptom index （CPSI） established by the National Institutes of Health （NIH） ： CPSI high score group and CPSI low group, bacterial infection group, and 30 healthy controls. CD64 expression of neutrophils in prostatic fluid was detected by flow cytometry and prostate fluid routine microscopy inspection.ResultsThe expression of CD64 in severe group （high score） was （4857.25±278.45） molecules/cell, which was significantly higher than that in the mild group （P〈0.05） . There was significant difference between the mild group and the non-bacterial infection group or the healthy control group （P〈0.01） . There was no significant difference between non-bacterial infection group and healthy control group. The expression of CD64 was positively correlated with some white blood cells in the bacterial infection group, but not the whole. ROC curve was drawn according to the data of the subjects. When CD64 was 2135.2 molecules/cell, the sensitivity was 92.9% and the specificity was 91.7%.ConclusionQuantitative detection of CD64 in prostatic fluid can be used as a sensitive index for diagnosis of chronic bacterial prostatitis and has clinical value.