Ghrelin基因多态性与原发性高血压患者危险因素的关系研究

The Relationship between Ghrelin Gene Polymorphism and Essential Hypertension in Patients with Risk Factors

目的:探讨人生长激素释放多肽(Ghrelin)基因多态性与原发性高血压患者危险因素的关系及其机制。方法:2014年10月至2015年10月选取80例原发性高血压患者进行研究,另选取80例健康体检者为对照组,采用Taq Man-PCR法测定两组Ghrelin基因多态性,并分析Ghrelin基因多态性与高血压患者高危因素的关系。结果:与对照组相比,高血压组患者CC、CA、AA型基因频率分布为68.75%、17.50%、13.75%,等位基因C、A频率分别为0.185、0.875,对照组分别为18.75%、35.00%、46.25%,等位基因C、A频率分别为0.125、0.815。Ghrelin基因CC型高血压患者血清甘油三脂(TC)、低密度脂蛋白(LDL-C)、总胆固醇(TG)、收缩压(SBP)、舒张压(DBP)、腰臀比(WHR)、体质量(BMI)水平显著高于CA型及AA型(P<0.05),而HDL-C低于CA型及AA型(P<0.05)。经卡方及Logistic多因素分析,Ghrelin基因CC型与TC异常、LDL-C异常、SBP异常、DBP异常、BMI异常有关。结论:Ghrelin基因多态性与导致高血压的高危因素,如TC异常、LDL-C异常、SBP异常、DBP异常、BMI异常等有关,因此,Ghrelin基因多态性可能参与原发性高血压的发病机制。

Objective： To investigate the relationship between the human growth hormone releasing peptide （Ghrelin） and the essential hypertension. Methods： 80 cases of patients with essential hypertension were studied and 80 cases of healthy were selected from October 2014 to October. The Ghrelin gene polymorphism assays of two groups were used with TaqMan-PCR method and Ghrelin gene polymorphism in patients with risk factors for hypertension were analyzed. Results： Compared with the control group, patients with hypertension CC, CA, AA genotype frequency distribution was 68.75%, 17.50%, 13.75%, allele C, A frequencies were 0. 185,0.875, the control group were 18.75% , 35.00%, 46.25%, allele C, a frequencies were 0. 125,0.815. Ghrelin gene CC type serum triglyeerides in patients with hypertension （TC） , low density lipoprotein （LDL- C） , total cholesterol （TG） , systolic blood pressure （SBP） , diastolic blood pressure （DBP） , waist-hip ratio （ WHR） , body mass （BMI） were significantly higher than those of CA and AA genotype （P 〈0.05） , whereas HDL-C and lower than the CA type AA type （ P〈0.05 ）. Chi square and Logistic multivariate analysis showed that the Ghrelin gene type CC was associated with TC abnormalities, LDL-C abnormalities, SBP abnormali- ties, DBP abnormalities, and BMI abnormalities. Conclusion： Ghrelin gene polymorphism and risk factors lead to high blood pressure, such as abnormal TC, LDL-C exception, SBP abnormality, DBP abnormality,BMI and abnormal, and therefore, Ghrelin gene polymorphism may be involved in essential hypertension pathogenesis.