电针肺与大肠经俞募穴改善急性胰腺炎大鼠胃肠动力减轻肺损伤的机理

Mechanism of Electro-acupuncture at Shu and Mu Acupoints of Lung and Large Intestine Meridians for Ameliorating the Gastrointestinal Motility to Reduce the Lung Injury Complicated with Acute Pancreatitis in Rats

目的:研究大承气汤作为基础治疗时,电针肺与大肠经俞募穴改善急性胰腺炎(AP)大鼠胃肠动力、减轻肺损伤的疗效及机理。方法:将48只大鼠随机分入假手术组(SG)、AP模型组(MG)、大承气汤组(A)、大承气汤+俞穴针刺组(B)、大承气汤+募穴针刺组(C)和大承气汤+俞募穴针刺组(D),每组8只。实验组大鼠均采用逆行性胆胰管注射法注入3.5%牛黄胆酸钠建立AP模型,SG组注入生理盐水。造模2 h后开始分别对B、C、D组大鼠进行3次针刺,每次间隔7 h留针40 min。造模24 h后大承气汤治疗组大鼠灌胃,给药12 h后处死大鼠,采集心血、肺和胰腺组织,计算肠推进率,取肺、胰腺做病理切片并进行病理评分,用酶联免疫法检测血清CCK、VIP、MTL及肺组织MPO、MDA。结果:与A组比较,C组血清CCK、MTL明显增加,VIP明显减少,肠推进率增加(P<0.05),肺组织MPO、MDA及病理评分均明显降低(P<0.05);B组仅CCK增加较明显(P<0.05),肺组织MPO及肺组织病理评分明显降低(P<0.05);D组仅MTL增加较明显(P<0.05),其它各项指标均无明显差异。结论:电针肺与大肠经俞募穴能通过调整血清CCK、MTL、VIP改善胃肠动力,减少肺组织MPO、MDA,减轻AP大鼠肺损伤,且募穴疗效更优。

Objective：To explore whether electro - acupuncture （EA） at Shu and Mu acupoints of lung and large intestine meridians could ameliorate gastrointestinal motility （GM）to reduce acute lung injury （ALI） complicated with AP in rats and its mechanism when Da Cheng Qi decoction （DCQD） was used as the fundamental treatment. Methods：Forty- eight rats were randomly divided into a sham- operation group （SG）, a model group （MG）, a DCQD group（ A）, DCQD ＆ Shu point group （B）, DCQD ＆ Mu point group （C） and DCQD ＆ Shu and Mu points group （D）, with eight rats in each group. Rats in the experimental groups were induced AP by retrograde perfusion into the biliopancreatic duct of 3.5 % sodium taurocholate, while group SG received normal saline instead. Two hours after injection, rats in group B, C, D were separately performed EA for three times,with the interval time of seven hours and retaining needles for forty minutes each time. Twenty - four hours after injection, rats in treatment groups were given DCQD intragastrically. Intestinal propulsion rates of all rats were measured after twelve hours of DCQD administration. Then they were sacrificed and their blood, lung and pancreas tissue samples were collected for pathological examination and CCK, VIP, MTL in blood as well as MPO and MDA were measured by ELISA. Results：Compared with Group A, CCK and MTL in group C were significantly increased and VIP was significantly decreased and intestinal propulsion rates were also increased （P 〈 0.05 ）. MPO ＆ MDA and pathology score of lung in group C were obviously lower （ P 〈 0.05 ） ; CCK in group B was obviously increased （ P 〈 0.05 ）. MPO and pathology score of lung in group B were obviously lower （P 〈 0.05 ）. MTL in group D was obviously increased （P 〈 0.05 ）. However, other indicators in group D were not significant. Conclusion：EA at Shu and Mu acupoints of lung and large intestine meridians can increase CCK ＆ MTL and decrease VIIi to ameliorate GM and lower MPO ＆ MDA to reduce the lung injury complicated with acute pancreatitis in rats. It shows that the Mu point is more effective.