摘要
目的:建立高效液相色谱法测定巴日嘎-13散中茜草有效成分大叶茜草素的含量。方法:Dikma Dimonsil C18色谱柱(250 mm×4.6 mm,5μm),流动相为甲醇-水-四氢呋喃(527∶90∶3),检测波长为391nm,流速为1 m L·min-1。结果:大叶茜草素在5.5~55μg·m L-1范围内呈良好的线性关系(r=0.9999),平均加样回收率为102.24%,RSD为1.48%。结论:本方法简便可靠、分离度较好、结果稳定,可用于巴日嘎-13散的质量检测。
Objective： To develop a method for determination of mollugin in a prepared Mongolia traditional medicine Bariga-13. Methods： HPLC method was built by using Dikma Dimonsil Diamonsil C18（250 mm×4.6 mm, 5μm） as column and methanol-water-tetrahydrofuran（527∶90∶3） as mobile phase. The detection wavelength was set at 391 nm. Results： The calibration curve of Mollugin was linear（ r=0.9999） in the range of 5.5-55 μg·m L-1. The average recovery was 102.24%; RSD was 1.48%. Conclusion： The method is simple, reproducible and reliable to produce good separation results. It can be applied to the quality test of Bariga-13.
出处
《中国药事》
CAS
2017年第2期178-181,共4页
Chinese Pharmaceutical Affairs
