盐酸二甲双胍磁性分子印迹聚合微球的合成

Preparation of magnetic molecularly imprinted polymer for metformin hydrochloride recognition

以盐酸二甲双胍为模板分子、Fe_3O_4为磁核、α-甲基丙烯酸为功能单体、乙二醇二甲基丙烯酸酯为交联剂、偶氮二异丁腈为引发剂,采用表面印迹聚合法成功制备了对盐酸二甲双胍具有特异性识别功能的磁性分子印迹聚合微球。合成的磁性聚合物具有良好的磁响应,在外加磁场下可实现快速磁分离。红外光谱、X射线光电子能谱和扫描电镜结果证实聚合物为核壳结构,磁性粒子由硅烷偶联剂完全包覆,并在其表面形成了一层分子印记聚合物。静态吸附实验结果表明,制备的磁性分子印迹聚合物对模板分子盐酸二甲双胍具有良好的吸附能力,其饱和吸附量为83mmol/kg。以双胍类降糖成分盐酸丁二胍和盐酸苯乙双胍为竞争分子,磁性印迹聚合物对模板分子表现出良好的特异选择性。

Magnetic molecularly imprinted polymer microspheres（MMIPs）were prepared by surface imprinted polymerization,using surface modified Fe3O4,metformin hydrochloride（MH）,ethylene glycol dimethacrylate（EGDMA）,methacrylic acid（MAA）and 2-methylpropionitrile（AIBN）as magnetic component,template molecules,crosslinker,functional monomer and initiator,respectively.The results of FT-IR and SEM showed that modified Fe3O4 dispersed well in the prepared magnetic molecularly imprinted polymers.MMIPs showed strong magnetic responsiveness and can be collected conveniently under the external magnetic field.The recognition capacity of MMIPs for metformin hydrochloride was investigated by equilibrium adsorption experiment.The binding amount of MMIPs was up to 83mmol/kg under the equilibrium state.Using buformin（BN）and phenformin hydrochloride（PH）as a structural analogue,the MMIPs had better adsorption affinity and selectivity for metformin hydrochloride than MNIPs.