共轭荧光聚合物复合物的高通量筛选及其微球的性能研究

Study on the high-throughput screening of conjugated fluorescent polymer composite and the performance of their beads

以丙烯酸酯/酰胺类单体为原料,采用喷墨打印技术分别将单体与荧光染料溶液打印到载玻片上,经紫外光引发进行原位聚合制得包载荧光共轭聚合物的聚合物微阵列芯片。采用高通量筛选法对聚合物微点的荧光强度进行研究,从而获得能使荧光强度增强或淬灭的共聚物。再采用反相悬浮聚合法将筛选出的聚合物制备成微球,通过红外、扫描电镜、热失重及荧光光谱对其进行结构、热稳定性能及荧光特性的表征。研究表明,甲基丙烯酸羟乙酯(HEMA)/乙二醇二甲基丙烯酸酯(EGMA)(HEMA/EGMA,质量配合比为5∶15)包载荧光染料后,在400℃时,失重率为45%;在25℃时,相对荧光强度达到42cd,热稳定性和光稳定性都得到了提高。

Polymer microarrays immobilized with a fluorescent conjugated polymer were prepared by inkjet printing solutions of acrylate/acrylamide monomers and the dye on glass slides respectively and subsequently polymerized in situ upon exposing to a UV light.The fluorescent images of spots were taken and analyzed to identify polymer candidates that could enhance the fluorescent intensity or quench the entrapped fluorescence.The beads of identified candidates were prepared by using reversed suspension polymerization method and characterized with FT-IR,SEM,TGA and fluorescence photometer.The results indicated that the thermo-and fluorescent stability of copolymer of 2-hydroxyethyl methacrylate（HEMA）∶ethylene glycol dimethacrylate（EGMA）（5∶15,ratio in weight）were improved by immobilization of the dye.The percentage of its weight loss was 45% at 400℃ and the relative fluorescence intensity was 42 cd at 25℃.