人肝癌细胞株的特性及其实验应用

Characteristics and experimental applications of human hepatocellular carcinoma cell lines

当前,原发性肝癌是我国高发的恶性肿瘤,死亡率高,治疗困难,对其研究一直是热点.然而,考虑到人道主义精神,肝癌相关研究无法直接在人体上进行.人肝癌细胞株模型的建立打破了这一瓶颈,其来源于人肝癌患者组织,具有一套完整的人体遗传基因,传代较稳定,是合适的实验对象.本文介绍了目前实验中常用的人肝癌细胞株SMMC7721、Bel-7402、MHCC97、HepG2、Hep3B、Huh-7、PLC/PRF/5的来源、特性,与肝癌细胞株的优势、作用、实验应用,希望能有助于该领域的研究.

At present, primary hepatocellular carcinoma（HCC）, a malignant tumor with a high incidence rate in China, is associated with a high mortality rate as well as a treatment dilemma. The research on HCC is always a hot topic. However, due to ethical considerations, research on HCC cannot directly be done in humans. The establishment of human HCC cell line model has overcome this obstacle. Derived from the tissue of human HCC, HCC cell lines have a complete set of human genes and relatively stable passages. This paper introduces the origins and characteristics of cell lines commonly used in experiments, such as SMMC7721, Bel-7402, MHCC97, Hep G2, Hep3 B, Huh-7, and PLC/PRF/5, as well as their roles and applications in HCC research.