微柱凝胶免疫分析技术检测新生儿免疫性血小板减少症自身抗体的效果

Effect of microcolumn gel immunoassay technology in detecting autoantibody of neonatal immune thrombopenia

目的观察微柱凝胶免疫分析技术(MGIA)检测新生儿免疫性血小板减少症(NITP)自身抗体的应用效果。方法应用MGIA和固相凝集法平行检测145例新生儿血小板减少患儿(及阳性患儿母亲)的自身抗体,比较两种方法检测结果的差异。结果 MGIA检测145例患儿自身抗体阳性14例,阳性率9.66%;固相凝集法检测阳性14例,阳性率9.66%,二者结果一致。MGIA检测14例阳性患儿母亲自身抗体的阳性率100.00%高于固相凝集法检测14例阳性患儿母亲自身抗体的阳性率57.14%,差异有统计学意义(P<0.05)。结论 MGIA方法对NITP自身抗体的检测灵敏度高,且操作简单、易于标准化,有利于NITP的鉴别诊断和早期制定针对性治疗方案。

Objective To observe the application effect of microcolumn gel immunoassay technology （MGIA） in detecting autoantibody of neonatal immune thrombopenia （NITP） . Methods MGIA and solid-phase agglutinate parallel detection method were used to detect autoantibodies of 145 neonates with NITP and their mothers, the differences of the two methods were compared. Results The positive rates of antibody in 145 neonates detected by MGIA and solid-phase agglutination method were 9. 66% （ 14 neonates）, the results of the two methods were the same. The positive rates of antibody in mothers of 14 neonates detected by MGIA was 100. 00% , which was higher than that by solid-phase agglutination method （57.14%） （P〈0. 05 ） . Conclusion The sensitivity of MGIA in detection of NITP autoantibody is high, the operation is simple and easy to be standardized, MGIA is conducive to differential diagnosis of NITP and early development of targeted treatment plan.