放射抗拒鼻咽癌细胞中Annexin A2相互作用蛋白的研究

Study on Annexin A2 Interacting Proteins in Nasopharyngeal Carcinoma Cells

目的筛选放射抗拒鼻咽癌细胞中Annexin A2的相互作用蛋白,以探讨Annexin A2在鼻咽癌放射抗拒中的作用机制。方法采用免疫共沉淀结合质谱(MALDI-TOF/TOF)技术筛选并鉴定放射抗拒鼻咽癌细胞CNE-2(R743)中Annexin A2相互作用蛋白,用Mascot软件在NCBInr数据库对质谱数据进行检索,生物信息学分析Annexin A2及其相互作用蛋白在放射抗拒鼻咽癌细胞中的作用。结果有5种蛋白与Annexin A2存在相互作用,分别是Calnexin、HSP90、GRP78、Vimentin、HSP27。将这些蛋白与Annexin A2进行生物信息学分析发现HSP90、GRP78和HSP27与Annexin A2存在直接相互作用,Calnexin、Vimentin与Annexin A2存在间接作用。结论这些蛋白可能与Annexin A2存在相互作用从而导致鼻咽癌细胞的放射抗拒。

Objective To investigate the mechanism of Annexin A2 in radioresistant nasopharyngeal carcinoma（NPC） cells by screening for Annexin A2 interacting proteins in radioresistant NPC cells. MethodsScreening and identification of Annexin A2 interacting proteins in radioresistant nasopharyngeal carcinoma CNE-2（R743） cells were done by co-Immunoprecipitation（Co-IP） combined with mass spectrometry（MALDI-TOF/TOF）. The mass spectra data were searched using NCBInr database with Mascot software. Analysis of the role of Annexin A2 and its interacting proteins in the radiation resistance of nasopharyngeal carcinoma cells by bioinformatics. Results Five proteins were interacting with Annexin A2, which were Calnexin, HSP90, GRP78, Vimentin, HSP27, respectively. Bioinformatics analysis of these proteins and Annexin A2 revealed that GRP78, HSP27 and HSP90 interact with Annexin A2 directly, but Calnexin, Vimentin interact with Annexin A2 indirectly. Conclusion These proteins may interact with Annexin A2 resulting in radiation resistance of nasopharyngeal carcinoma cells.