HGT KAPs基因家族在辽宁绒山羊皮肤毛囊中的表达研究

The Expression of Glycine/Tyrosin-rich Keratin-associated Proteins Genes in Hair Follicle of Liaoning Cashmere Goat

为进一步探索高甘氨酸/酪氨酸角蛋白关联蛋白基因家族(HGT KAPs)与辽宁绒山羊羊绒品质——绒毛纤维直径的关系,本研究运用半定量RT-PCR检测KAP6-1.2、KAP6.2、KAP7.1、KAP8.1和KAP8.2在皮肤及各组织中的表达,通过原位杂交对其进行定位分析,采用实时荧光定量PCR检测兴盛期、退行期基因的相对表达量。结果表明:5个基因特异地表达于皮肤,在初、次级毛囊的皮质层、内根鞘中有明显的表达信号。KAP6-1.2和KAP8.1在兴盛期初次级毛囊的表达量差异不显著,KAP6.2在兴盛期、退行期初级毛囊的表达量均高于次级毛囊,而KAP7.1、KAP8.2在兴盛期次级毛囊的表达量高于初级毛囊,而退行期与之相反。因此推测,HGT KAPs基因家族在调控辽宁绒山羊绒毛纤维直径方面具有重要的作用,是编码羊绒结构蛋白的基因。

This experiment was conducted to study the relationship between Glycine/Tyrosin-rich Keratin-associated Proteins Genes（HGT KAPs） and cashmere fiber quality（cashmere fiber diameter） of Liaoning cashmere goat. The semiquantitative RT-PCR was used to detect whether genes of KAP6-1.2, KAP6.2, KAP7.1, KAP8.1 and KAP8.2 were expressed in skin and other organs, in situ hybridization was used to detect the genes expression location,the real-time quantitative PCR was used to analyze the relative quantitative of genes during hair follicle anagen and catagen. The results showed that the genes were only present in skin, the relative quantitative of genes had different result during hair follicle anagen and catagen.In conclusion, HGT KAPs genes play an important role in controlling cashmere fiber diameter of Liaoning cashmere goat.