火龙果对大鼠生长的试验研究

Experimental study on growth in rats by feeding pitaya

目的观察"紫红龙"火龙果对SD大鼠30 d喂养的生长影响。方法根据实验动物体重,将80只SD大鼠按体重随机分为4组,每组20只,雌雄各半。实验设为阴性对照组和低、中、高3个剂量组,火龙果剂量分别为:7.5、10.0、15.0 g/kg bw。各剂量组每天灌胃不同浓度的火龙果果浆,阴性对照组灌胃同体积的蒸馏水,连续灌胃30 d。试验期间每周称量进食量和体重,计算食物利用率;试验结束时,股静脉取血进行动物的血常规检查、血液生化指标检查,称量脏器重量,计算脏器系数,对主要脏器进行病理组织学检查。结果试验周期内各试验组动物生长良好,体重逐周增长正常,未见明显中毒体征,动物无死亡。试验结束时各血液生化指标均在本实验室正常参考值范围,血常规指标与对照组比较差异均无统计学意义(P>0.05)。各试验组动物的主要脏器重量、组织病理检查未发现异常。结论 "紫红龙"火龙果对SD大鼠生长期内的各项观察指标未见毒性影响。

Objective To investigate the effect of ＂Hylocereus polyrhizus＂pitaya on Sprague Dawley（ SD） rats. Methods Random grouping experiment designed by weight was used to evaluate the effects of ＂ Hylocereus polyrhizus＂ pitaya. 80 SD rats（ male and female in half） were exposed to pitaya（ 0 g/kg,7. 5 g/kg,10 g/kg and 15 g/kg） by oral perfusion at gradient doses for thirty day respectively. Body weight,food intakes were measured every week. The haematological,biochemical parameters and organ coefficient were measured at the end of experiment,the histopathological examination was also performed. Results In the test period,the growth of each test group animal was good,the the growth of weight was normal by week,no obvious signs of poisoning,no animal died. The difference of normal blood routine indexes and the control group had no statistical significance（ P〉0. 05）. There were no adverse effect observed,including body weight,food intake,food consumption,hematology,serum biochemistry,as well as histopathology. Conclusion There is no toxic signs of ＂Hylocereus polyrhizus＂pitaya on SD rats.