不同品种猪在不同生理阶段骨骼肌葡萄糖转运载体和胰岛素受体表达的发育性变化

Ontogenetic Regulation of Glucose Transporter and Insulin Receptor mRNA Expression in Skeletal Muscle of Different Kinds of Pigs at Different Physiological Stages

本试验旨在研究不同品种猪在不同生理阶段骨骼肌葡萄糖转运载体(GLUT)1、GLUT4和胰岛素受体(IR)mRNA表达的发育规律。试验以1、7、30、60、90和150日龄蓝塘和长白两品种猪背最长肌、半膜肌和半腱肌组织样品c DNA为模板,采用实时荧光半定量PCR的方法,检测猪GLUT1和GLUT4 mRNA在不同骨骼肌中的表达模式。结果显示:1)1~150日龄蓝塘和长白猪背最长肌、半膜肌和半腱肌GLUT1 mRNA表达存在相似的发育变化规律,在1日龄时相对表达丰度最高。2)1~150日龄蓝塘和长白猪半膜肌和半腱肌GLUT4 mRNA相对表达丰度随日龄呈波形式变化,变化速度和程度存在品种差异。1日龄和150日龄时蓝塘猪背最长肌GLUT4mRNA相对表达丰度低于同日龄长白猪(P>0.05),而在其余日龄时高于同日龄长白猪(P>0.05)。3)蓝塘和长白猪背最长肌、半膜肌和半腱肌IR mRNA相对表达丰度在1和30日龄时表现出较高水平,而在7、60、90和150日龄时无显著差异(P>0.05);同日龄蓝塘和长白猪之间IR mRNA相对表达丰度无显著性差异(P>0.05)。结果表明:猪骨骼肌GLUT1 mRNA的表达在不同生理阶段存在差异,猪骨骼肌GLUT4 mRNA的表达在不同品种和生理阶段存在显著差异,且其与IR mRNA的表达相关性较弱,无明显规律。

This experiment was conducted to determine the ontogenetic regulation of glucose transporter（GLUT） 1,GLUT4 and insulin receptor（IR） mRNA expression in skeletal muscle of different kinds of pigs at different physiological stages.Lantang（LT） and Landrace（LD） pigs at the age of 1,7,30,60,90 and150 days were selected to determine the expression patterns of GLUT1 and GLUT4 at different skeletal muscle tissues including longissimus dorsi muscles（LM）,semimembranosus muscles（SM） and semitendinosus muscles（SD） by the method of real-time semiquantitative PCR for cDNA templates.The results showed as follows：1） LT and LD pigs exhibited similar development of mRNA expressions of GLUT1 in the LM,SM and SD from 1 to 150 days of age with the highest level at the age of 1 day.2） LT and LD pigs exhibited waveform variations of mRNA relative expression abundance of GLUT4 from 1 to 150 days of age with breed differences in the speed and extend of variations.At the age of 1 and 150 days,the mRNA relative expression abundance of GLUT4 in the LM of LT pig was lower than that of LD pig with the same age（P〉0.05）,while was higher than that of LD pig with the same age of the rest days（P〉0.05）.3） LT and LD pigs exhibited higher level of the mRNA relative expression abundance of IR in the LM,SM and SD at the age of 1 and 30 days,while there were not significant difference among 7,60,90 and 150 days of age（P〉0.05）.There was no significant difference in mRNA relative expression abundance of IR between LT and LD pigs with the same ages（P〉0.05）.The results suggest that GLUT1 mRNA expression in skeletal muscles of pigs can be regulated by physiological stages,while GLUT4 mRNA expression is not only differentially regulated by breeds but also differentially distributed in physiological stages,and the expression level of GLUT4 is weakly related to the IR mRNA expression and shows no apparent rules.