摘要
本研究旨在研究茶皂素对奶牛瘤胃原虫区系的影响。选取12头健康荷斯坦奶牛[体重(550±30)kg,产奶量35 kg/(头·d),胎次2~4],随机分为4组,每组3头,均饲喂基础饲粮,分别于晨饲前灌服0(对照)、20、30、40 g/(头·d)的茶皂素。试验期49 d,其中预试期14 d,正试期35 d,正试期内每7 d于晨饲前1 h用口腔采样器采集瘤胃液。采用聚丙烯酰氨凝胶电泳(DGGE)结合18S r DNA序列分析技术研究瘤胃原虫区系的变化。结果表明:1)添加茶皂素可以选择性地抑制瘤胃中原虫的生长。2)与对照组相比,30、40 g/(头·d)茶皂素组的丰富度指数和香农多样性指数都显著降低(P<0.05),优势度指数显著增加(P<0.05),但是均一性指数无显著变化(P>0.05);茶皂素显著减少了瘤胃中前庭亚纲内毛目的原虫数量。综上所述,饲粮中添加30、40 g/(头·d)茶皂素均可抑制奶牛瘤胃原虫的生长,并且降低瘤胃原虫的多样性。
The objective of this study was to research the effects of tea saponin on rumen protozoa flora of dairy cows.Twelve healthy Holstein dairy cows[body weight was(550±30) kg) milk yield was35 kg/(head·d);2 to 4 parities]were randomly allocated to one of four groups with 3 cows per group.Tea saponin[0(control),20,30 and 40 g/(head·d)]was perfused to cows before morning feeding.The experiment lasted for 49 d with 14 d of adaptation period and 35 d of formal test period.Rumen fluid was collected by mouth sampler at 1 h before morning feeding every 7 d during formal test period.Denaturing gradient gel electrophoresis(DGGE) combined with 18 S rDNA sequence analysis technique was used for rumen protozoa flora analysis.The results showed as follows:1) tea saponin could selectively inhibit protozoa growth.2)Compared with control group,richness index and Shannon index in 30 and 40 g/(head·d) tea saponin groups were significantly reduced(P〈0.05),and dominance index was significantly increased(P〈0.05),but evenness index was not significantly different among groups(P〉0.05);tea saponin could significantly reduce rumen Vestibuliferia Entodiniomorphida protozoa number.In conclusion,dietary supplementation of 30 and40 g/(head·d) tea saponin can inhibit protozoa growth in rumen of dairy cows,and reduce the diversity of rumen protozoa.
出处
《动物营养学报》
CAS
CSCD
北大核心
2017年第2期620-627,共8页
CHINESE JOURNAL OF ANIMAL NUTRITION
基金
“十二五”国家科技支撑项目(25012BAD14B09)
“十三五”国家重大科技专项(2016YFDO70020,2016YFDO700205)
北京市农业局北京市现代农业产业技术体系奶牛创新团队
关键词
奶牛
原虫区系
茶皂素
dairy cow
protozoa flora
tea saponin