妊娠后期营养限制对母羊胃肠道葡萄糖转运载体相关基因表达的影响

Effects of Nutritional Restriction during Late Gestation on Gene Expressions of Glucose Transporters in Gastrointestinal Tract of Ewes

本研究旨在研究妊娠后期营养限制对母羊胃肠道葡萄糖转运载体相关基因表达的影响。选取20只同期受孕的湘东黑山羊,随机分为2组,即对照组(自由采食)和限饲组(40%采食量限制),每组10只。预试期15 d(妊娠81~95 d),正试期39 d(妊娠96~135 d)。正试期结束后,屠宰并采取瘤胃、十二指肠、空肠、回肠以及盲肠的黏膜样品,利用实时定量PCR技术,检测Na+-葡萄糖共转运载体1(SGLT1)、Na+-葡萄糖共转运载体3(SGLT3)、易化葡萄糖转运载体2(GLUT2)和易化葡萄糖转运载体5(GLUT5)基因表达量。结果表明:限饲组与对照组相比,SGLT1基因表达量在瘤胃显著降低(P<0.05),在空肠和回肠中有降低趋势(0.05≤P<0.10);GLUT5基因表达量在盲肠显著降低(P<0.05);而其他葡萄糖转运载体基因胃肠道表达量在限饲组和对照组差异均不显著(P>0.05)。由此可见,母羊妊娠后期营养限制对胃肠道中葡萄糖转运载体基因表达有不同程度的影响,进而引起母羊机体内葡萄糖转运的改变。

This experiment was conducted to investigate the effects of nutritional restriction during late gestation on gene expressions of glucose transporters in gastrointestinal tract of ewes.Twenty Xiangdong black pregnant goats were chose and randomly assigned to 2 groups：control group（ad libtum） and restricted group（40%restriction of feed intake）,and each group had 10 ewes.The pre-trial lasted for 15 d（81 to 95 d of gestation）,and the trial lasted for 39 d（96 to 135 d of gestation）.After the trial,ewes were slaughtered to collected raucosa samples of rumen,duodenum,jejunum,ileum and cecum.Real-time PCR analysis was used to determine the gene expression levels of sodium/glucose cotransporter member 1（SGLT1）,sodium/glucose cotransporter member 3（SGLT3）,facilitated glucose transporter member 2（GLUT2） and facilitated glucose transporter member 5（GLUT5）.The results showed as follows：compared with control group,the expression level of SGLTl gene in rumen of restricted group was significantly decreased（P〈0.05）,and there was a decreased tendency for that in jejunum and ileum（0.05≤P〈0.10）.The expression level of GLUT5 gene was significantly decreased in cecum（P〈0.05）;while the expression levels of other glucose transporter genes in gastrointestinal tracts were not significantly different between restricted group and control group（P〉0.05）.Collectively,nutritional restriction has different effects on the expressions of glucose transporter genes located on gastrointestinal tracts,thereby leading to the changes of glucose transport.