谷胱甘肽的高效液相色谱及色谱联用检测方法进展

Application of HPLC and hyphenated chromatograph on Glutathione measurement

谷胱甘肽(glutathione,GSH)是一种含有巯基的三肽化合物。机体内谷胱甘肽主要以还原型的状态存在,氧化应激后形成氧化型谷胱甘肽(oxidized glutathione,GSSG),后者在谷胱甘肽还原酶的作用下再形成还原型谷胱甘肽(reduced glutathione,GSH)。还原型谷胱甘肽具有保护细胞免遭氧化损伤的重要作用。几乎所有哺乳动物体内都有谷胱甘肽。研究机体内还原型谷胱甘肽以及氧化型谷胱甘肽的含量,可以评价机体的氧化应激情况和疾病发生的风险。虽然很多学者都对体内的氧化型谷胱甘肽和还原型谷胱甘肽进行了检测,但是检测结果差异较大。这是由于GSH发生自氧化从而造成GSH的低估,及GSSG的高估和(或)GSH/GSSG的低估。本文主要对GSH的色谱以及色谱联用例如:液质联用(liquid chromatograph-mass spectrometer,LC-MS)等方法进行综述。

Glutathione（GSH） is a thiol-containing tripeptide. Free glutathione is present mainly in its reduced form, which can be converted to the oxidized glutathione（GSSG） during oxidative stress, and can be reverted to the reduced form by the action of the enzyme glutathione reductase（GSH）.Reduced glutathione plays an essential role in protecting cells from oxidative damage. Almost all mammals have glutathione in their bodies Measurement of GSH and GSSG is a useful indicator of oxidative stress and disease risk. As a possible marker of oxidative stress, many studies have measured GSH and GSSG. However, large differences in GSH and GSSG levels reported in different studies, calls for a reliable standardized method. This is due to the autoxidation of GSH resulting in an underestimation of GSH, and the overestimation of GSSG and（or） GSH/GSSG underestimation In this paper, we review determination GSH and GSSG with high performance liquid chromatography（HPLC）and hyphenated chromatography such as liquid chromatographmass spectrometer（LC-MS）,et al.