茶树菇凝集素AAL、AAL-2检测小鼠CD8^+T淋巴细胞表面糖基化研究

Glycosylation of CD8^+ T Lymphocytes from C57BL/6 Mice Recognized by Agrocybe aegerita Lectins

本研究使用两种大型真菌茶树菇新型凝集素AAL(Agrocybe aegerita lectin)和AAL2(Agrocybe aegerita lectin 2),以检测小鼠CD8^+T淋巴细胞表面糖基化。用生物素标记的AAL和AAL2结合流式细胞仪,分别分析从C57BL/6小鼠脾脏和腹股沟淋巴结分离出来的CD8^+T淋巴细胞表面的糖基化水平,及其细胞因子表达水平。结果发现脾脏中AAL^+CD8^+,AAL2^+CD8^+T淋巴细胞所占的比例(%)分别为6.72±3.00和12.18±5.28,而腹股沟淋巴结的AAL^+CD8^+,AAL2^+CD8^+T淋巴细胞所占的比例(%)分别为12.18±5.28和10.15±3.46。细胞因子表达水平结果发现小鼠脾脏AAL2^+CD8^+T细胞表达perforin的细胞比例低于AAL2-CD8^+T细胞(p<0.05,有统计学意义),腹股沟淋巴结中AAL^+CD8^+T细胞表达perforin的细胞比例则高于AAL-CD8^+T细胞(p<0.05,有统计学意义)。该数据为研究小鼠脾脏和腹股沟淋巴结CD8^+T细胞表面糖基化提供了新的数据,也为两种新型凝集素AAL和AAL2的应用提供理论依据。

Two novel lectins, AAL（Agrocybe aegerita lectin） and AAL2（Agrocybe aegerita lectin 2）, were employed to detect the glycosylation of CD8~＋ T lymphocytes from C57BL/6 mouse spleen and inguinal lymph nodes. Biotinylated AAL and AAL2, combined with flow cytometry, were used to analyze the glycosylation of CD8~＋ T lymphocytes and cytokine expression. The proportion（%） of AAL~＋CD8~＋ and AAL2~＋CD8~＋ T lymphocytes in spleen was found to be 6.72±3.00 and 12.18±5.28, respectively. The proportion（%） of AAL~＋CD8~＋ and AAL2~＋CD8~＋ T lymphocytes in lymph nodes was 12.18±5.28 and 10.15±3.46, respectively. Moreover, the frequency of perforin~＋ cells in spleen AAL2~＋CD8~＋ T cells was lower than that in AAL2-CD8~＋ T cells（p0.05）, and the frequency of perforin~＋ cells in lymph node AAL~＋CD8~＋ T cells was higher than that in AAL-CD8~＋ T cells（p0.05）. This study provides new data on the glycosylation of spleen and inguinal lymph node CD8~＋ T cells and the application of two new lectins, AAL and AAL2.