表面等离子体共振免疫传感器用于快速检测微囊藻毒素的研究

Research of the surface plasmon resonance immunosensor for rapid detection of microcystin

针对现有微囊藻毒素(MC-LR)检测方法操作复杂、因标记而污染环境、仪器贵重,不利于现场快速检测等问题,将自行研制的表面等离子体共振(SPR)生物芯片检测仪应用于微囊藻毒素的检测,提出抑制型SPR生物芯片快速检测痕量微囊藻毒素的方法。采用该方法分别对浓度为3.5、2.5、1.5、1、0μg/L的MC-LR样品进行了检测。结果表明:该方法检测限小于1μg/L,可满足世界卫生组织(WHO)对于饮用水和我国地表水环境质量标准中MC-LR最低含量检测的需求。该方法完成一个样品检测耗时约8 min,相比于高效液相色谱法(HPLC)和酶联免疫吸附法(ELISA)等传统检测方法,快速定量是其最大的优势,可用于食品质量监控和现场实时检测。

As the existing pesticide detection methods were complicated, would pollute the environment by marking, with precious instrument,and not conducive to on-site rapid detection, the self-developed surface plasmon resonance biological chip detector was used in the detection of microcystin（MC-LR） , a method for rapid detection of microeystin by inhibiting type SPR biological chip was proposed.The competitive inhibition immunoassay was preceded in M C-LR with concentrations of 3.5, 2.5,1.5,1,0 μg/L.The experimental results showed that the detection limit could be 1 μg/L which could meet the needs of the World Health Organization （WHO）for detecting the minimum content of MC- LR in drinking water and surface water environmental quality standards in China.The detection of 1 sample would take about 8 min by this method,compared with the traditional methods such as high performance liquid chromatography （HPLC）and enzyme linked immunosorbent assay （ELISA） ,the rapid and quantitative was the biggest advantage of this method.The established device and method could be used for food quality monitoring and on-site real-time detection.